by Madalina Diaconu, Business Development Manager, EW Nutrition

Modern poultry production is currently battling a perfect storm of respiratory, enteric, and bacterial pressures. These overlapping challenges do more than just make birds sick; they actively erode performance, lead to higher condemnation rates at the plant, and squeeze already tight profit margins. To stay ahead, any practical health program must move beyond quick fixes and instead align interventions across everything from gut integrity and immunity to farm management and data collection.

Despite significant technological leaps in biosecurity and disease control, many “old” enemies remain stubbornly persistent:

• Coccidiosis: This remains the single largest financial drain on the industry, costing an estimated EUR 10.4 billion globally due to losses in weight gain and increased mortality. (Blake et al., 2020)
• Necrotic Enteritis (NE): Often triggered by coccidiosis, NE ranges from “silent” subclinical performance loss to sudden, fatal outbreaks. (Hargis, 2024; Skinner et al., 2010)
• Histomoniasis: In turkeys, this disease (often called Blackhead) frequently results in 80-100% mortality, made worse by the fact that there are currently no approved treatments in major markets. (Beer et al., 2022; Merck, 2024)
• APEC/Colibacillosis: This is a major driver of bird loss and processing plant condemnations, complicated by a high prevalence of multi-drug resistance. (Apostolakos et al., 2021; Joseph et al., 2023; Kazimierczak et al., 2025)
• Salmonella: This pathogen persists at critical production nodes, with varying strains moving through the production pyramid from breeders to the final product. (Siceloff et al., 2022)

Why a pillar-based approach?

Pillar 1: Pathogen pressure & epidemiology

Respiratory pathogens like IBV or NDV often show up as mixed infections, leading to high morbidity and more condemnations. MG and MS amplify these chronic issues. (Liu et al., 2025; El-Gazzar, 2025; CFSPH) Enteric pathogens like Eimeria (coccidiosis) create the groundwork for Clostridium perfringens (NE) to thrive. (Blake et al., 2020; Hargis, 2024; Skinner et al., 2010)

• The Phytogenic Lever: Essential oils and plant polyphenols can disrupt the membranes of bacteria like Salmonella and E. coli, lowering the overall intestinal load and reducing environmental shedding. (Gentile et al., 2025; Wickramasuriya et al., 2022)

Pillar 2: Immunity & Vaccination

Successful vaccination isn’t just about the bottle; it requires precise strain selection, prime/boost design, and correct application. This is especially true for managing AIV (Avian Influenza) under global risk-based strategies. (FAO/WOAH, 2025)

• The Phytogenic Lever: Certain plant-based additives act as immunomodulators, boosting macrophage activity and helping birds maintain resilience even when stressed by high stocking densities or heat. (Wickramasuriya et al., 2022)

Pillar 3: Microbiome & Gut Integrity

“Dysbacteriosis” is essentially a microbiome out of balance, which ruins nutrient absorption and weakens the gut barrier. (Aruwa et al., 2021; Aruwa & Sabiu, 2024) Protecting the gut is essential because clinical NE can kill birds quickly, while subclinical NE silently ruins efficiency. (Hargis, 2024; Skinner et al., 2010)

• The Phytogenic Lever: These additives support “good” bacteria like Lactobacilli while suppressing opportunists and strengthening the “tight junctions” in the gut lining. (Wickramasuriya et al., 2022) Multiple trials show reduced NE pressure when phytogenics accompany coccidiosis programs. (Wickramasuriya et al., 2022)

Pillar 4: Environment & Management

The environment plays a massive role; for instance, recycling litter beyond six cycles significantly increases the risk of Salmonella detection. (Machado et al., 2020) Proper ventilation is also key to preventing thermal stress, which can trigger gut dysbiosis. (Liu et al., 2025; Aruwa et al., 2021)

• The Phytogenic Lever: By stabilizing digestion and the microbiota, these additives can reduce wet litter and ammonia release, indirectly improving respiratory comfort. (Wickramasuriya et al., 2022; Aruwa et al., 2021)

Pillar 5: Biosecurity & Movement Control

Disease spreads through networks. Prioritizing biosecurity at “high-centrality” nodes – like hatcheries and common service routes – is more effective than a blanket approach. (Sequeira et al., 2025)

• The Phytogenic Lever: Reducing the amount of pathogens a flock sheds helps support structural biosecurity barriers by lowering the overall transmission risk within houses. (Gentile et al., 2025; Wickramasuriya et al., 2022)

Pillar 6: Water, Feed & Processing Interface

Water hygiene is a vital tool for microbiome stability, especially during the vulnerable brooding phase. (Wickramasuriya et al., 2022) At the processing plant, PAA chillers remain the most effective chemical intervention to reduce contamination. (Thames et al., 2022)

• The Phytogenic Lever: Using phytogenics in feed or water helps stabilize the upper-GI tract during feed transitions and can lower carcass pathogen loads. (Gentile et al., 2025; Wickramasuriya et al., 2022)

Pillar 7: Diagnostics, Genomics & Data Systems

Modern tools like Whole Genome Sequencing (WGS) and RT-PCR panels allow for much faster detection of APEC or respiratory viruses, enabling “precision” interventions. (Kazimierczak et al., 2025; El-Gazzar, 2025; Liu et al., 2025)

• The Phytogenic Lever: When data shows rising pathogen pressure, phytogenics offer a flexible, rapid-response alternative that helps maintain antibiotic stewardship. (Kazimierczak et al., 2025; Gentile et al., 2025)

A 12-Month Roadmap for Implementation

• Q1: Baseline & Risk Map: Map pathogen pressure using targeted PCR/WGS panels and review movement networks to prioritize high-centrality nodes. (Kazimierczak et al., 2025; El-Gazzar, 2025; Liu et al., 2025; Siceloff et al., 2022; Sequeira et al., 2025)
• Q2: Program Design: Update vaccine strains and set up co-management plans for coccidiosis and NE, including microbiome supports with clear targets. (Liu et al., 2025; El-Gazzar, 2025; Blake et al., 2020; Hargis, 2024; Wickramasuriya et al., 2022)
• Q3: Execution & Plant Linkage: Solidify water/feed hygiene SOPs and link farm Salmonella trends to plant PAA chiller performance. (Siceloff et al., 2022; Thames et al., 2022; Sequeira et al., 2025)
• Q4: Review & Scale: Audit how well the team followed the diagnostic-driven actions and refine the playbooks for the next cycle. (Kazimierczak et al., 2025)

The Integrated View

Phytogenic feed additives aren’t “silver bullets,” but they contribute across all seven pillars. Their multi-target mode of action – acting as anti-inflammatories, antioxidants, and antimicrobials – complements traditional tools like vaccines and biosecurity to build a more resilient bird. (Wickramasuriya et al., 2022; Gentile et al., 2025; Aruwa et al., 2021)

References

Apostolakos I, et al. Occurrence of colibacillosis and APEC population structure in broilers. Front Vet Sci (2021). https://www.frontiersin.org/articles/10.3389/fvets.2021.737720/full

Aruwa CE, et al. Poultry gut health – microbiome functions and engineering. J Anim Sci Biotechnol (2021). https://link.springer.com/article/10.1186/s40104-021-00640-9

Aruwa CE, Sabiu S. Interplay of poultry–microbiome interactions and dysbiosis. British Poultry Science (2024). https://www.tandfonline.com/doi/pdf/10.1080/00071668.2024.2356666

Beer LC, et al. Histomonosis in poultry: a comprehensive review. Front Vet Sci (2022). https://www.frontiersin.org/articles/10.3389/fvets.2022.880738/full

Blake DP, et al. Re‑calculating the cost of coccidiosis in chickens. Veterinary Research (2020). https://link.springer.com/article/10.1186/s13567-020-00837-2

CFSPH. Avian Mycoplasmosis (MG) Fact Sheet (updated). https://www.cfsph.iastate.edu/Factsheets/pdfs/avian_mycoplasmosis_mycoplasma_gallisepticum.pdf

El‑Gazzar M. Mycoplasma gallisepticum infection in poultry. MSD Veterinary Manual (rev. 2025). https://www.msdvetmanual.com/poultry/mycoplasmosis/mycoplasma-gallisepticum-infection-in-poultry

FAO/WOAH. Global Strategy for HPAI Prevention and Control (2024–2033). (2025). https://www.woah.org/app/uploads/2025/02/web-gf-tads-hpai-strategy-woah.pdf

Gentile N, et al. Emerging challenges in Salmonella control: innovative, sustainable disinfection strategies in poultry farming. Pathogens (2025). https://www.mdpi.com/2076-0817/14/9/912

Hargis BM. Necrotic enteritis in poultry. Merck Veterinary Manual (rev. 2024). https://www.merckvetmanual.com/poultry/necrotic-enteritis/necrotic-enteritis-in-poultry

Joseph J, et al. APEC in broiler breeders: an overview. Pathogens (2023). https://www.mdpi.com/2076-0817/12/11/1280

Kazimierczak J, et al. Rapid detection of APEC via minimal virulence markers (iroC, hlyF, wzx ‑ O78). BMC Microbiology (2025). https://link.springer.com/article/10.1186/s12866-025-03861-4

Liu H, et al. Review of respiratory syndromes in poultry: pathogens, prevention, and control measures. Veterinary Research (2025). https://link.springer.com/content/pdf/10.1186/s13567-025-01506-y.pdf

Machado PCJ, Chung C, Hagerman A. Modeling Salmonella spread in broiler production: determinants and control strategies. Front Vet Sci (2020). https://www.frontiersin.org/articles/10.3389/fvets.2020.00564/full

Merck Vet Manual. Histomoniasis in poultry (rev. 2024). https://www.merckvetmanual.com/poultry/histomoniasis/histomoniasis-in-poultry

Pant S, et al. Economic impact assessment and disease prevalence of coccidiosis in broilers. Journal of Entomology and Zoology Studies (2019). https://www.entomoljournal.com/archives/2019/vol7issue5/PartK/7-4-112-882.pdf

Sequeira SC, et al. Livestock & poultry movement networks for disease surveillance/control. PLOS One (2025). https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0328518

Siceloff AT, Waltman D, Shariat NW. Regional Salmonella differences in U.S. broiler production (2016–2020). Applied and Environmental Microbiology (2022). https://journals.asm.org/doi/10.1128/aem.00204-22

Skinner JT, et al. An economic analysis of subclinical necrotic enteritis in broilers. Avian Diseases (2010). [suspicious link removed]

Thames HT, et al. Prevalence of Salmonella and Campylobacter at processing; PAA efficacy. Animals (2022). https://www.mdpi.com/2076-2615/12/18/2460

Wickramasuriya SS, et al. Role of physiology, immunity, microbiota and infectious diseases in poultry gut health. Vaccines (2022). https://www.mdpi.com/2076-393X/10/2/172

by Ilinca Anghelescu, Global Director Marketing & Communications, EW Nutrition

2025 was a year defined by four converging forces for the global feed and animal production industry: an unprecedented HPAI crisis that cost American consumers alone $14.5 billion extra in egg expenditures; historic record corn production driving feed ingredient prices lower; a highly disruptive US tariff regime that reshuffled global trade flows for soybeans, corn, chicken, and pork; and accelerating regulatory pressure on antimicrobial use across Europe and globally.

The strategic imperatives from 2025 are clear: biosecurity investment is no longer optional, ingredient price volatility demands agile procurement strategies, trade compliance is a weekly operational concern, and antibiotic-free production transitions require credible, phased plans now.

KEY METRIC: Global chicken meat production reached approximately 105 million MT in 2025 (+2%), even as egg production suffered severely. The global feed market is valued at $542 billion in 2025, growing at 3.3% CAGR. Corn hit record production of 17 billion bushels in the US alone – the highest since 1936 in terms of harvested area.

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CHAPTER 1: HPAI & DISEASE LANDSCAPE 

1.1  The Ongoing H5N1 Crisis – Scale & Impact

The H5N1 clade 2.3.4.4b strain of Highly Pathogenic Avian Influenza (HPAI) continued to dominate animal health headlines in 2025. Since its reemergence in February 2022, the US outbreak alone has resulted in the confirmed loss of over 175 million birds across 1,700+ flocks – the costliest poultry disease event in recorded history.

1.2  2025-Specific Developments

United States: Early-Year Severity, Policy Response

The first six weeks of 2025 saw 28 million layers depopulated – the worst start to any calendar year on record. Ohio, Indiana, and Missouri bore the brunt. The USDA launched a five-pronged approach in February 2025 including:

• Gold-standard biosecurity assessments (948 completed Jan 20–June 26)
• Indemnity increase from $7 to $17 per lost layer hen
• Importation of 26+ million dozen shell eggs from Brazil, Honduras, Mexico, Turkey, and South Korea
• Removal of select regulatory burdens to accelerate flock repopulation
• $793 million in HPAI research proposals received in response to USDA Innovation Grand Challenge

⚠  Price Manipulation Investigation: In April 2025, the DOJ Antitrust Division launched an investigation into the largest US egg producer after it reported a 247% increase in quarterly net income. Egg producers and retailers face ongoing scrutiny over whether crisis pricing exceeded what supply constraints warranted.

Brazil: First Commercial HPAI Outbreak – May 2025

On May 15, 2025, Brazil – the world’s largest poultry exporter, responsible for nearly 30% of global exports – confirmed its first-ever commercial HPAI case at a breeder facility in Montenegro, Rio Grande do Sul (17,000 birds). This was a watershed event for global poultry trade.

Europe: Persistent Pressure

HPAI continued to circulate widely in European poultry and wild bird populations. Key 2025 events include recurrence in Australia (February), ongoing outbreaks in Germany, Hungary, Netherlands, UK, and France, and the first confirmed domestic cat HPAI death in the Netherlands (H5N1, November 2025).

CRITICAL RISK: HPAI is now classified as enzootic (endemic) in wild birds across North America by the CDC. The virus circulates year-round in wildlife reservoirs, making seasonal recurrence in commercial flocks a structural, not episodic, risk. US egg producers are 8% below their 2022 flock baseline.

1.3  Other Priority Diseases in 2025

CHAPTER 2: GLOBAL POULTRY PRODUCTION 

2.1  Global Output – 2025 Performance

Despite HPAI disruptions, global chicken meat production grew approximately 2% in 2025 to around 105 million MT (ready-to-cook), driven by demand resilience and lower feed costs for broiler production. Total global poultry meat (including turkey, duck, and others) is forecast to exceed 152 million MT for 2025, per FAO Food Outlook June 2025.

OECD-FAO 10-Year Outlook (2025–2034)

The OECD-FAO Agricultural Outlook 2025–2034, released in July 2025, projects global poultry meat production will grow by over 19% to 173.4 million MT by 2034 compared to the 2022–24 average. Poultry will account for the majority of additional meat consumption globally, driven by:

• Affordability relative to beef and pork, especially in price-sensitive emerging markets
• Population and income growth in Southeast Asia, South Asia, and Sub-Saharan Africa
• Rapid urbanization and expansion of Quick Service Restaurant (QSR) chains
• Superior feed conversion ratio (FCR) and lower greenhouse gas emissions per kg of protein

STRATEGIC NOTE: In high-income countries, per capita poultry consumption growth is flattening as consumers focus increasingly on welfare, environment, and health attributes. Growth opportunity is almost entirely in middle-income markets. Product premiumization (antibiotic-free, cage-free, organic) is the North American and European story.

2.2  Egg Production – Crisis Sector

Egg production was the sector hardest hit by HPAI globally. In the US, 75% of all HPAI-affected birds were table-egg layers, despite layers comprising less than 4% of the total poultry population. This structural vulnerability reflects longer flock lifespans and, increasingly, cage-free housing adoption.

⚠  Cage-Free Transition & Disease Vulnerability: Some analysts link cage-free housing to higher HPAI susceptibility. Regardless of epidemiological debate, the US cage-free market is now structurally undersupplied relative to corporate commitments made in 2014–2017. Producers face a squeeze: comply with welfare commitments while managing disease risk.

CHAPTER 3: FEED INGREDIENT MARKETS 

3.1  Grain & Oilseed Prices – 2025 Summary

From a feed cost perspective, 2025 was broadly favorable for livestock and poultry producers. Record US corn production and generally adequate global grain and oilseed supplies put downward pressure on the major feed commodities, offering partial relief from the margin pressure of recent years.

PROCUREMENT SIGNAL: The US/China trade tensions created windows of soybean buying opportunity as prices swung on trade deal news. China agreed to purchase US soybeans in late 2025 as part of a limited trade deal, causing a price uptick. Procurement teams should monitor US-China negotiations as a lead indicator for soybean pricing in 2026.

3.2  Global Feed Market Overview

3.3  Key Ingredient Trends to Watch

Fertilizer Cost Relief

Fertilizer prices have declined significantly from their 2022 peak. A basket of N, P, and K fertilizers averaged $437/tonne in May 2025, down from the $815/tonne peak in April 2022, per FAO Food Outlook. This benefits grain production economics and should support adequate grain supplies into 2026.

Soybean Oil Competition: Biodiesel vs. Feed

US soybean oil demand from renewable fuel programs (the 45Z credit) competed directly with feed-grade fat supplies, pushing soy oil prices up 20.8% in 2025. Feed mills formulating with added fats should evaluate alternative lipid sources. Poultry fat and palm olein remain cost-competitive in some markets.

Alternative Proteins: Insect Meal, DDGS, Algae

While adoption remains limited in volume, regulatory acceptance of insect meal in EU poultry diets continues to expand. Dried Distillers Grains with Solubles (DDGS) remain a strategically important co-product, particularly in the US and EU. Feed formulators should have up-to-date matrix values and be prepared to use them when corn prices favor inclusions.

⚠  Tariff Risk for Feed Inputs: US feed manufacturers faced effective tariff rates averaging 12%+ on key agricultural inputs from China and other countries in 2025, including herbicides, pesticides, and some micro-ingredient precursors. Amino acid supplies (predominantly Chinese-origin lysine, methionine, threonine) faced added cost and supply uncertainty.

CHAPTER 4: TRADE POLICY DISRUPTIONS 

4.1  The 2025 US Tariff Regime – Agricultural Impact

The Trump administration’s tariff policies beginning January 20, 2025, represented the most significant disruption to global agricultural trade in decades. The three largest US agricultural export markets – Mexico ($30.3B in 2024), Canada ($28.3B), and China ($24.7B) – were all targeted, triggering retaliatory measures that hit feed, grain, poultry, and pork exports.

CHINA TRADE DEAL (MAY 2025): A 90-day tariff truce agreed May 12, 2025 reduced US tariffs on Chinese goods from 145% to 30%, and China’s tariffs on US products from 125% to 10%. China agreed to purchase US soybeans. No permanent deal was signed. The limited agreement provided short-term stability but medium-term uncertainty remains.

4.2  Impact on US Agricultural Trade Flows

4.3  Strategic Trade Lessons

• Supply chain diversification is no longer a luxury: concentration of US soy exports to China created a single-point-of-failure vulnerability that became fully exposed in 2025.
• Regionalized disease zoning is a trade-preserving tool: Brazil’s rapid implementation of regionalized HPAI bans (rather than country-wide) preserved most of its export access; this is the model the industry should support with regulators globally.
• USMCA dependency is real: 70% of US corn, 60% of soybeans, 45% of poultry exports go to Mexico, Canada, China – the same three countries targeted by 2025 tariffs.
• US government announced $12B in emergency farm compensation in 2025, repeating the pattern from Trump’s first term – indicating persistent trade disruption risk.

CHAPTER 5: REGULATORY CHANGES 

5.1  EU: Feed & Food Safety Legislation Simplification

In 2025, the European Commission proposed a package to streamline EU food and feed safety legislation while maintaining high health standards. The initiative, announced mid-2025, is intended to boost competitiveness of EU producers by reducing regulatory complexity – a direct response to competitive concerns vs. non-EU producers.

5.2  EFSA 2025 Guidance on Microorganisms

On September 24, 2025, EFSA’s Scientific Committee adopted new harmonized guidance on the characterization of microorganisms in the food chain. This is a landmark shift with major implications for feed additive manufacturers, probiotics suppliers, and novel food applicants.

5.3  Antimicrobial Resistance (AMR) – Regulatory Pressure

AMR remains the defining long-term regulatory risk for the animal feed and production industry. Key 2025 actions:

• EFSA/ECDC Joint Report (March 2025): Highlighted persistently high resistance to critical antimicrobials in poultry, especially Campylobacter and Salmonella, with ‘statistically significant increasing trend 2020–2024.’ This directly fuels EU legislative pressure.
• EU Regulation 2019/6 (Veterinary Medicines) – Article 118: Banning import of animal products containing antimicrobials used for growth promotion. Application delayed to 2026, raising questions about enforcement timelines – and competitive fairness regarding imports from countries still allowing AGPs.
• EU AMR Implementation Decision 2023: New harmonized monitoring requirements for AMR in zoonotic and indicator bacteria from food-producing animals – effective January 1, 2025. All EU Member States now required to collect and report standardized AMR surveillance data.
• WOAH 10-Year HPAI Strategy (2024–2033): Promotes surveillance, vaccination programs, and timely reporting as cornerstones of international HPAI management.

BOTTOM LINE ON AMR: The regulatory trajectory is clear and irreversible – sub-therapeutic antibiotic use for growth promotion is being eliminated globally. The timeline varies by region (already banned in EU since 2006; US voluntary approach from 2017; global WHO action plan). Companies that have already invested in transition are ahead; those that have not face increasing compliance risk and market access restrictions.

5.4  US Regulatory Developments

CHAPTER 6: FEED ADDITIVE & NUTRITION STRATEGIES 

PRECISION NUTRITION SIGNAL: The industry’s shift to reduced crude protein (CP) diets, precisely supplemented with industrial amino acids (L-Lys, DL-Met, L-Thr, L-Trp, L-Val) remained the dominant reformulation strategy in 2025. Lower CP diets reduce feed cost, lower N excretion (environmental benefit), and reduce substrate for pathogenic bacteria. With amino acid prices remaining favorable, there are few economic arguments for maintaining high CP diets.

6.1  The Post-AGP Transition: Where the Industry Stands

The antibiotic-free (ABF) production movement accelerated further in 2025. With the EU ban on AGPs in place since 2006 and the US moving toward voluntary phase-out, the entire industry is in active transition. The key challenge: AGP removal creates enteric health gaps that must be addressed with alternative tools. Without effective management, removal of AGPs leads to increased necrotic enteritis, Campylobacter colonization, and poorer FCR.

6.2  Heat Stress – A Growing Production Challenge

Climate-related heat stress was a highlighted research and production topic in 2025. Modern high-performance broiler genetics have been selectively bred for rapid growth under thermoneutral conditions. Heat stress impairs feed intake, FCR, immunity, meat quality, and reproduction. Management strategies:

• Dietary electrolyte balance adjustment (increase K, Na, reduce Cl where appropriate)
• Vitamin C and E supplementation at heat stress periods
• Betaine inclusion as an osmolyte; reduces supplemental methionine requirement under heat stress
• Feed schedule adjustment (limit feeding during hottest hours; early morning/evening feeding)
• Housing design investment: tunnel ventilation, evaporative cooling, adequate air velocity

6.3  In Ovo Technology

In ovo vaccination and nutrition delivery continued to advance in 2025. Key developments include high-throughput systems (3,000 eggs/hour at 99% accuracy) for in ovo vaccination and nutritional interventions. Early-life gut programming through in ovo delivery of probiotics, nutrients, and vaccine antigens is becoming an increasingly important hatchery-level biosecurity and performance tool.

CHAPTER 7: MARKET TRENDS & CONSUMER SHIFTS 

7.1  Poultry Gaining Share vs. Other Proteins

Elevated beef prices throughout 2025 – driven by tight US cattle supply (herd at decades-long lows) and high demand – continued to push consumers toward poultry as a cost-effective protein. This dynamic is a structural tailwind for the broiler industry globally.

7.2  Cage-Free & Animal Welfare Commitments

The cage-free transition is structurally undersupplied in the US. Corporate commitments made in 2014–2017 implied a need for 220 million cage-free layers by 2025–26. Current production is well below that target. This creates both a market opportunity (premium pricing) and a risk (HPAI vulnerability concerns in cage-free systems). Producers must balance welfare compliance with biosecurity protocols.

7.3  Antibiotic-Free, Organic, and Specialty Products

Consumer and corporate buyer demand for ABF, No Antibiotics Ever (NAE), organic, and pasture-raised products continued to grow in premium markets in 2025. The pasture-raised egg segment reported 30% annual growth rates despite high price points. For integrated producers, this requires dedicated production lines with separate management protocols, supply chain segregation, and robust documentation systems.

7.4  Sustainability Pressure

Feed manufacturers and integrators are under growing pressure from retail and foodservice customers, NGOs, and regulators to demonstrate reduced environmental footprint. Key metrics under scrutiny:

• GHG emissions per kg of chicken meat produced (Scope 1, 2, and 3)
• Deforestation-free supply chains for soy (EU Deforestation Regulation – EUDR)
• Feed conversion ratio improvement as a sustainability lever
• Nitrogen and phosphorus excretion reduction (enzyme use, reduced CP diets, phytase)
• Water use per unit of animal protein produced

EUDR NOTE: The EU Deforestation Regulation requires companies to ensure that soy used in feed does not originate from recently deforested land. Implementation deadlines have been debated, but traceability requirements for soy origin – particularly from Brazil – are operationally significant for EU feed manufacturers and importers.

CHAPTER 8: STRATEGIC LESSONS & ACTION PRIORITIES 

8.1  Summary: Top 10 Lessons of 2025

8.2  Action Priority Matrix for Management Teams

8.3  Key Indicators to Monitor in 2026

• HPAI detection frequency in fall-winter 2025–26 migration season – predictor of next egg price cycle
• USDA HPAI vaccine grand challenge awards – signals timeline for commercial vaccine availability
• EU feed safety simplification package progress – potential relief on additive authorization timelines
• EUDR deforestation enforcement timeline – soy traceability compliance clock
• Brazil HPAI market re-entry for China – recovery of the world’s #1 poultry export relationship
• US corn/soy 2026 planting intentions (March) – USDA Prospective Plantings report is the key 2026 procurement signal

2025 demonstrated that the feed and animal production industry operates in an environment of simultaneous, compounding risks – biological, geopolitical, regulatory, and climatic. The companies that performed best were those with robust biosecurity infrastructure, agile procurement teams, clear AMR transition roadmaps, and diversified market exposure. There is no single silver bullet. Systematic risk management, not reactive crisis response, is the competitive differentiator going forward.

  KEY SOURCES & REFERENCES 

This article draws on data and analysis from the following sources:

Author: Ajay Bhoyar, Senior Global Technical Manager, EW Nutrition

The global use of feed enzymes has become a central feature of efficient monogastric animal production systems. Rising feed ingredient costs, tighter margins, and increasing regulatory pressure to reduce environmental impact have all accelerated enzyme innovation. At the same time, feed mills have shifted toward higher conditioning temperatures and time in pursuit of improved pellet durability, pathogen control, and throughput. However, this creates a hostile environment for most exogenous feed enzymes, which can lose significant activity under the harsh conditions of feed processing.

Historically, enzyme manufacturers have attempted to overcome heat degradation of by coating, encapsulating, or post-pelleting liquid application (PPLA) of enzymes. While these approaches provide partial solutions, they can also have limitations, including delayed enzyme activity, uneven distribution, reduced mixing uniformity, and reliance on specialized liquid enzyme applicators.

These limitations prompted a novel direction: enzymes designed or selected to be intrinsically heat-stable, capable of surviving pelleting without protective matrices.

This article highlights recent advancements in intrinsically heat-stable xylanase technology, explains its advantages over coated and post-pelleting enzyme solutions, and outlines its practical benefits for feed manufacturers, integrators, and nutritionists operating under modern high-temperature feed pelleting conditions.

Intrinsically Thermostable Enzymes

An enzyme is considered intrinsically heat-stable when its native protein structure resists unfolding and retains catalytic activity under high temperatures associated with feed processing—typically 80–95°C for 30–90 seconds. Unlike coated enzymes that rely on external protection, intrinsically thermostable enzymes depend on their internal protein architecture for heat tolerance. Enzymes from organisms living in compost, thermal springs, and geothermal soils naturally withstand temperatures of 80–100 °C or higher. Intrinsically thermostable enzymes are often sourced from thermophiles (organisms living in hot springs and deep-sea vents) or engineered for stability. They resist denaturation (loss of shape and function) at high-temperature processing.

Limitations of Current Thermostability Solutions

Coating / Encapsulation

A method of protecting enzymes from heat is to encapsulate or coat them with a protective coating. An ideal enzyme coating for animal feed needs to:

1. Protect the enzyme through steam conditioning (typically 85–90°C or higher) and through subsequent pelleting.

2. Release the enzyme from the coating quickly in the gastrointestinal tract of the target animal, to ensure optimum efficacy. (Gilbert and Cooney, 2007)

There is some evidence, however, suggesting that the coating of enzymes may reduce the efficacy of the product, compared to an uncoated version of the same product (Kwakkel et al., 2000).

Post-Pelleting Liquid Application (PPLA)

Post-pelleting liquid enzyme application requires sophisticated applicators to minimize the risk of uneven spraying or calibration errors, which is often not feasible in small or mid-size mills. Accurate application of the liquid enzyme, as with some other critical liquid micro-ingredients, requires specialized spraying equipment and, even then, consistency of accurate enzyme application can be an issue (Bedford and Cowieson, 2009). Research has shown that as much as 30% of the enzyme activity can be found in the pellet fines, and therefore, adding the enzyme before screening would result in a lower than expected dosage in the final feed and wastage of the enzyme product (Engelen, 1998). In some cases, adjusting the pelleting machines to the output of the PPLA’s spray nozzles to ensure a homogenous and even application of the enzyme on the pellets may reduce the overall pellet production rate, especially in big feed mills with very high throughput.

These limitations of the coated or PPLA technologies strengthen the value proposition of intrinsically heat-stable enzymes.

Nutritional and Commercial Benefits of Intrinsically Heat-Stable Xylanase

The use of intrinsically heat-stable xylanase delivers consistent nutritional benefits in poultry and swine feeds, including predictable non-starch polysaccharide (NSP) degradation, a significant increase in the metabolizable energy (ME) value of the feed, and enhanced gut health resilience supporting reduced antibiotic use.

From a commercial and operational perspective, this technology simplifies enzyme application, improves mixing uniformity, reduces formulation risk, and lowers feed cost per unit of meat or egg produced.

In-Vitro Thermal Stability Profile of Axxess XY

Axxess XY is a novel, intrinsically thermostable GH10 xylanase originating from Thermotoga maritima, a hyperthermophilic bacterium found in hydrothermal vents near volcanic grounds, and commercially it is produced by proprietary strain of Bacillus subtilis.

The superior heat stability of Axxess XY has been proven under various commercial pelleting conditions across different geographies. Axxess XY showed excellent post-pelleting recovery under commercial feed-milling conditions across varying temperatures and conditioning times (Fig. 2).

In one study, in addition to excellent post-pelleting recovery, Axxess XY also demonstrated high xylanase stability in pelleted feed over a 2-month feed storage period at>40°C, with humidity around 65%.

Conclusions

As feed mills continue to operate at higher conditioning temperatures and longer retention times, enzyme heat stability has become a critical success factor in modern feed production. Intrinsically heat-stable xylanase offers a practical and reliable solution to this challenge by maintaining enzyme activity through pelleting without the need for coatings or post-pelleting liquid application systems.

By relying on its native protein structure rather than external protection, intrinsically thermostable xylanase delivers consistent post-pelleting recovery, uniform distribution in feed, and predictable nutritional performance across different feed mills and processing conditions. This reliability translates into improved nutrient utilization, better gut health support, and reduced cost per kilogram of meat or eggs produced.

From an operational standpoint, intrinsically heat-stable xylanase simplifies enzyme application, reduces dependence on specialized equipment, and minimizes the need for over-formulation or safety margins. These advantages help feed manufacturers and integrators improve efficiency, lower risk, and achieve more consistent results, especially under demanding commercial pelleting conditions.

In summary, intrinsically heat-stable xylanase aligns well with the evolving needs of today’s feed industry, offering a robust, cost-effective, and future-ready enzyme solution for high-performance animal production systems.

References:

Bedford, M. R., and A. J. Cowieson. 2009. “Phytate and Phytase Interactions.” In Proceedings of the 17th European Symposium on Poultry Nutrition, 7–13. Edinburgh, UK.

Eeckhout, M., M. De Schrijver, and E. Vanderbeke. 1995. “The Influence of Process Parameters on the Stability of Feed Enzymes during Steam Pelleting.” In Proceedings of the 2nd European Symposium on Feed Enzymes, 163–169. Noordwijkerhout, The Netherlands.

Engelen, G. M. A. 1998. Technology of Liquid Additives in Post-Pelleting Applications. Wageningen, The Netherlands: Wageningen Institute of Animal Science.

Gilbert, T. C., and G. Cooney. 2011. “Thermostability of Feed Enzymes and Their Practical Application in the Feed Mill.” In Enzymes in Farm Animal Nutrition, 2nd ed., edited by M. R. Bedford and G. G. Partridge, 249–259. Wallingford, UK: CABI.

Kwakkel, R. P., P. L. van der Togt, and K. A. B. M. Holkenborg. 2000. “Bio-Efficacy of Two Phytase Formulations Supplemented to a Corn–Soybean Broiler Diet.” In Proceedings of the 3rd European Symposium on Feed Enzymes, 63–64. Noordwijkerhout, The Netherlands.

By Ilinca Anghelescu, Dr. Andreas Michels, Predrag Persak

Our understanding of how nutrition influences growth and resilience in poultry has greatly expanded in recent years. It is now clear that animal performance stems to a large extent from a balance between metabolism, immune function, and the gut microbiome. These systems interact continuously, and even small nutritional or environmental changes can shift the animals’ physiological response. This growing knowledge has encouraged the development of nutritional strategies and feed components that work through adaptive, non-antibiotic mechanisms. One recent proposed explanation for these responses has rapidly gained ground: hormetic modeling.

Hormetic modeling describes how small or moderate doses of nutritional components can activate beneficial adaptive responses (improved resilience or metabolic efficiency), while excessive doses become harmful. This idea parallels, largely speaking, Paracelsus’s famous principle: “The dose makes the poison.” In poultry nutrition, such hormetic patterns are well recognized in nutrients like trace elements (selenium, zinc) and specific amino acids (for example, arginine). At optimal levels, these nutrients support antioxidant defense, growth, and immune balance, whereas excessive intake may cause oxidative or metabolic stress

This review examines the hormetic principle and its application to modern poultry/swine feeding concepts, exploring how balanced nutrient design and controlled inclusion of bioactive compounds can strengthen cellular adaptation, improve stress tolerance, and enhance production efficiency.

How do AGPs actually work?

Despite AGP’s widespread historical use, the precise mechanisms by which subtherapeutic doses of antibiotics enhance animal productivity remained poorly understood. Recent advances in systems biology and mitochondrial research propose new answers, much needed to develop future advanced nutritional systems.

The traditional explanations for AGP efficacy have focused primarily on antimicrobial effects:

• reducing nutrient competition from microorganisms
• decreasing harmful bacterial metabolites
• improving gut wall morphology (thinner gut wall ➡ better nutrient absorption)
• preventing subclinical infections

However, these mechanisms alone could not fully explain why different classes of antibiotics with diverse mechanisms of action produce similar growth-promoting effects (Gutierrez-Chavez et al., 2025).

Niewold (2007) hypothesized that the primary mechanism of AGPs is non-antibiotic anti-inflammatory activity, reducing the energetic costs of chronic low-grade inflammation. Inflammation diverts nutrients from growth toward immune responses, with cytokine production (particularly IL-1β, IL-6, and TNF-α) suppressing anabolic pathways (Kogut et al., 2018). AGPs appear to selectively inhibit pro-inflammatory cytokine production without completely suppressing immune function.

A paper published in 2024 by Fernandez Miyakawa et al. proposes that antibiotics at subtherapeutic levels act primarily through mitochondrial hormesis and adaptive stress responses, and not simply through antimicrobial activity. In this model, mitochondria act as bioenergetic hubs and signaling centers. Low-dose antibiotics trigger mild mitochondrial stress, which triggers the activation of adaptive protective pathways.  This in turn induces mitokine release, leading to systemic adaptive responses improving growth, feed efficiency, and disease tolerance.

Mechanism of action in the hormetic model of AGP efficiency

Hormesis is a biphasic mechanism whereby high doses are toxic, but low doses stimulate adaptive responses and are beneficial. In the case of AGPs, Fernandez Miyakawa et al. propose that low doses stimulate growth, stress resistance, and cellular repair.

Key signaling pathways

As Bottje et al. (2006, 2009) shows, efficient animals often have mitochondrial inner membranes that are less permeable to protons and other ions, allowing for more effective coupling between electron transport and ATP synthesis, which reduces energy loss through proton leak and maximizes the production of ATP per oxygen molecule consumed. Lower membrane permeability is influenced by factors like decreased membrane surface area per protein mass, specific membrane protein content (such as adenine nucleotide translocase), and fatty acid composition in the membrane phospholipids, all contributing to a tighter barrier that prevents unregulated electron or proton flow and supports higher energetic efficiency. Such features make mitochondria in efficient species more capable of maintaining membrane integrity and ATP generation, especially when facing environmental stress, as seen in freeze-tolerant animals whose mitochondria do not undergo damaging permeability transitions under extreme conditions.

Nrf2

Many AGPs interfere with mitochondrial protein synthesis and electron transport chain. At subtherapeutic levels, they cause a mild ROS increase, which triggers the activation of redox-sensitive transcription factor Nrf2. Since Nrf2 regulates over 250 antioxidant, detoxification, and anti-inflammatory genes, the result is improved cell survival, redox balance, and tolerance to stress.

Mitokine production

Mitokines are “signaling molecules that enable communication of local mitochondrial stress to other mitochondria in distant cells and tissues” (Burtscher 2023). Through fibroblast growth factor 21 (FGF21), growth differentiation factor 15 (GDF15), adrenomedullin2 (ADM2) etc, these stress signals are released systemically and coordinate tissue-wide responses, leading to improved growth and resilience.

Inflammation and disease defense

While the negative side of antibiotic growth promoters is well researched and understood (Rahman et al., 2022), science can advance by isolating the positive effects and attempting to offer different pathways to the same benefits. One such lesson can be derived from understanding inflammation pathways and responses.

Chronic low-grade intestinal inflammation is common in modern poultry production, due to diet, microbiota shifts, high metabolic demands etc. This inflammation diverts energy from growth to immune responses.

AGPs reduce the energy costs of this inflammation in three main ways:

• Reduces inflammation through adaptive stress response
• Raising the threshold to trigger inflammation
• Promoting overall resilience, rather than simply killing pathogens

Fernandez Miyakawa et al. suggest, in this emerging model, that disease defense can operate two different actions: resistance to health challenges through reduction of the pathogen load (which is driven by the immune system and is energy costly); and overall resilience by reducing host damage without reducing the pathogen load. AGPs, the authors claim, mainly promote resilience by enhancing mitochondrial stress responses and tissue repair, i.e. more precisely:

• Direct mitochondrial stimulation in intestinal epithelial cells
• Systemic mitokine signaling coordinating organism-wide adaptive responses
• Selective microbiota modulation enhancing beneficial host-microbe interactions
• Improving resilience without immune system costs
• Metabolic optimization supporting growth and feed efficiency

In this context, “metabolic optimization” refers to the enhancement of metabolic processes within livestock or poultry to support efficient growth, feed conversion, and physiological resilience, without relying on immune-mediated pathways that are energetically costly. Scientific evidence shows that metabolic optimization involves improving nutrient assimilation, promoting more efficient energy production in tissues (such as mitochondrial ATP synthesis), and minimizing wasteful metabolic byproducts, resulting in reduced feed intake per unit of growth and better utilization of dietary nutrients (Rauw 2025, El-Hack 2025).

Function of feed additives and feed components

Feed additives and feed components in many ways represent the complete other side of the spectrum from antibiotics, but are there some features where antibiotics and feed additives come close in their functions? There is a good case to be made for certain feed additives ultimately working in the animal to achieve similar benefits to the desirable, non-medicinal usage of AGP´s. Especially with the emergent model of AGP mechanism described above, it is worth discussing how certain feed additives can support the same end goal: promoting animal resilience.

Lillejhoj et al (2018), Gutierrez-Chavez et al. (2025) and others outline the end-results such products must achieve:

• Growth performance & feed conversion efficiency
• Promotion of animal productivity under real-world conditions
• Support gut homeostasis
• Non-adverse effect on the immune system
• Reduction of oxidative stress
• Support organism in mitigation of enteric inflammatory consequences

Within the hormetic model, possibly the most important systemic benefit is, in one phrase, promoting resilience. Phytomolecules have long been used, in human and animal medicine, for the same end goal. The mechanisms described below should naturally be seen with caution, as phytomolecule microbiome effects can be subtler and context-dependent. However, the substantiating literature has been increasingly accumulating on these specific topics.

1. Immunometabolic regulation

Phytomolecules demonstrate remarkably similar anti-inflammatory effects to what Niewold (2007) suggested was a primary mechanism of AGPs: non-antibiotic anti-inflammatory activity, reducing the energetic costs of chronic low-grade inflammation. Inflammation diverts nutrients from growth toward immune responses, with cytokine production (particularly IL-1β, IL-6, and TNF-α) suppressing anabolic pathways (Kogut et al., 2018). AGPs appear to selectively inhibit pro-inflammatory cytokine production without completely suppressing immune function. A similar effect can be observed with various types of phytomolecules, which significantly reduced pro-inflammatory and/or increased anti-inflammatory cytokine expression in animals challenged with several pathogens. The anti-inflammatory mechanism appears to involve inhibition of NF-κB activation and modulation of MAPK signaling pathways (Kim et al., 2010; Long et al., 2021).

2. Mitochondrial hormesis and energy metabolism

Fernández Miyakawa et al. (2024, see above) proposed that AGPs exert growth-promoting effects through mitochondrial hormesis – subtherapeutic antibiotic doses induce mild mitochondrial stress, triggering adaptive responses that enhance mitochondrial function, energy metabolism, and cellular resilience. This mechanism, while requiring further validation, explains why different antibiotics with diverse targets produce similar growth outcomes.

The mitochondrial stress response involves activation of the IL-6 receptor family signaling cascade, which regulates metabolism, growth, regeneration, and homeostasis in liver and other tissues (Perry et al., 2024). Subtherapeutic antibiotic exposure activates proteins involved in growth and proliferation through IL-6R gp130 subunit signaling, including JAK, STAT, mTOR, and MAPK pathways.

Phytomolecules demonstrate similar mitochondrial effects. Perry et al. (2024) showed that increased activity of AMPK, mTOR, PGC-1α, PTEN, HIF, and S6K can also be available via phytomolecule activity, suggesting enhanced anabolic metabolism.

Capsicum oleoresin supplementation in broilers increased jejunal lipase and trypsin activity, enhanced ileal amylase activity, improved jejunal morphology, and modulated immune organ development, indicating enhanced digestive efficiency and nutrient utilization (Li et al., 2022).

Compounds such as vanillin, thymol, eugenol have been shown to improve glucose and lipid metabolism through TRPV1 activation and mitochondrial function enhancement (Gupta et al., 2022; Zhang et al., 2017).

3. Gut microbiota modulation

AGPs selectively reduce specific microbial populations, particularly Lactobacillus species that produce bile salt hydrolase (BSH). Since BSH reduces fat digestibility and thus weight gain, AGP-mediated reduction of BSH-producing bacteria enhances energy extraction and growth (Lin, 2014; Bourgin et al., 2021).

Recent research by Zhan et al. (2025) using single-molecule real-time 16S rRNA sequencing demonstrated that therapeutic antibiotic doses (lincomycin, gentamicin, florfenicol, benzylpenicillin, ceftiofur, enrofloxacin) significantly altered chicken gut microbiota composition, with Pseudomonadota and Bacillota becoming dominant phyla after exposure. Different antibiotics produced distinct temporal effects on microbial diversity and community structure.

Phytomolecules exert targeted antimicrobial effects while promoting beneficial bacteria. Dietary supplementation with 800 mg/kg Capsicum extract in Japanese quails reduced cecal counts of pathogenic bacteria (Salmonella spp., E. coli, coliforms) while modulating Lactobacilli populations (Reda et al., 2020).

In pigs, 80 mg/kg natural capsicum extract increased cecal propionic acid and total volatile fatty acid concentrations, with increased butyric acid in the colon – indicating enhanced fermentation by beneficial bacteria (Long et al., 2021).

Capsicum and Curcuma oleoresins altered intestinal microbiota composition in commercial broilers challenged with necrotic enteritis, reducing disease severity through microbiome modulation (Kim et al., 2015).

Capsaicin demonstrates selective antimicrobial activity, inhibiting pathogenic Gram-negative bacteria while favoring development of certain Gram-positive bacteria. The antibacterial mechanism involves induction of osmotic stress and membrane structure damage (Adaszek et al., 2019; Rosca et al., 2020).

4. Intestinal barrier function and gut health

AGPs have been associated with improved intestinal morphology, including increased villus height and reduced crypt depth, which enhance absorptive capacity (Gaskins et al., 2002).

Phytomolecules produce similar or superior effects. Capsicum extract (80 mg/kg) in pigs increased ileal villus height and upregulated MUC-2 gene expression, indicating enhanced gut barrier function and integrity. The improved barrier function correlated with reduced diarrhea incidence (Liu et al., 2013; Long et al., 2021).

Allium hookeri extract increased expression of tight junction proteins (claudins, occludins, ZO-1) in LPS-challenged broiler chickens, demonstrating direct enhancement of barrier integrity (Lee et al., 2017).

5. Oxidative stress mitigation

Oxidative stress impairs growth by damaging cellular components and triggering inflammatory responses. AGPs reduce oxidative stress indirectly through anti-inflammatory effects and microbiota modulation (Bortoluzzi et al., 2021).

Phytomolecules possess direct antioxidant properties. Capsicum extract (50 mg/kg) in heat-stressed quails reduced serum and ovarian malondialdehyde (MDA) while increasing superoxide dismutase (SOD) and catalase (CAT) activities. Ovarian transcription factors showed decreased NF-κB and increased Nrf2 and HO-1 expression (Sahin et al., 2016).

A mixture of herbal extracts including pepper reduced thiobarbituric acid reactive substances and MDA in broiler liver and muscle, while increasing glutathione peroxidase (GSH-Px) activity and improving antioxidant enzyme expression (Saleh et al., 2018).

Capsicum extract (80 mg/kg) in pigs increased total antioxidant capacity, SOD, and CAT while reducing MDA levels, demonstrating robust antioxidant effects (Long et al., 2021).

Standardization and controlled release: Critical success factors

A major criticism of phytomolecules has been inconsistent efficacy across studies. However, this variability largely reflects differences in:

• Active compound concentrations
• Bioavailability and stability
• Dosing precision
• Product quality and standardization

Microencapsulation is one of the technologies that address the standardization and bioavailability challenges. It protects volatile compounds from degradation during feed processing and storage, with encapsulated essential oils showing significantly higher retention compared to unprotected forms (Stevanović et al., 2018). By creating a protective barrier around active ingredients, microencapsulation enables controlled release in specific regions of the gastrointestinal tract, improving absorption efficiency and reducing dose variability (Bringas-Lantigua et al., 2011). The technology also masks unpalatable flavors that can reduce feed intake while standardizing active ingredient concentrations through precise manufacturing processes (Gharsallaoui et al., 2007). Studies demonstrate that spray-dried microencapsulated essential oils achieve encapsulation efficiencies exceeding 93% with minimal loss during storage (Hu et al., 2020), and can be engineered for enzyme-mediated release to ensure bioactive delivery at optimal intestinal sites (Elolimy et al., 2025).

Mechanistic synthesis: An integrated model

The evidence indicates that both AGPs and phytomolecules operate through an integrated network of effects:

1. Primary Level: Selective antimicrobial effects modify gut microbiota composition
2. Secondary Level: Reduced microbial metabolites (ammonia, endotoxins) decrease inflammatory signaling
3. Tertiary Level: Reduced inflammation conserves energy for growth; enhanced barrier function improves nutrient absorption
4. Quaternary Level: Mitochondrial hormesis and metabolic optimization increase energy efficiency
5. Systemic Level: Improved immunometabolic homeostasis supports optimal growth

This integrative model explains why multiple antibiotics with different mechanisms produce similar growth outcomes: they converge on common pathways regulating immunometabolism and mitochondrial function (Fernández Miyakawa et al., 2024).

Phytomolecules operate through the same mechanistic framework but with potential advantages:

• Multiple bioactive compounds providing redundancy
• Antioxidant effects enhancing stress resilience
• Lower AMR selection pressure
• Potential prebiotic-like effects supporting beneficial microbiota

Safety and antimicrobial resistance considerations

Antibiotic exposure significantly disrupts gut microbiota diversity and stability, with effects persisting beyond withdrawal periods. The study by Zhan et al. (2025) demonstrated that different antibiotics produce varying degrees of microbiota disruption, with florfenicol and gentamicin showing the strongest and most persistent effects.

In contrast, phytomolecules generally do not generate resistance through the same mechanisms as antibiotics. Some phytochemicals may actually enhance antibiotic efficacy and resensitize resistant bacteria through structural modifications of bacterial membranes (Khameneh et al., 2021; Suganya et al., 2022).

However, one study reported increased correlation between antibiotic resistance genes (ARGs) and mobile genetic elements in pig feces after mushroom powder supplementation, suggesting that certain phytogenic compounds may increase ARG mobility (Muurinen et al., 2021). This emphasizes the need for continued surveillance of phytomolecule effects on resistance gene dynamics.

Capsaicinoids and capsinoids have well-established safety profiles. Capsiate, a non-pungent analogue of capsaicin, exhibits substantially lower toxicity while maintaining similar metabolic and growth-promoting effects (Gupta et al., 2022). No adverse effects on animal health or product quality have been reported at recommended dosages in reviewed studies.

Future directions and research needs

Despite substantial progress, several areas require further investigation:

1. Mechanistic refinement: Detailed characterization of signaling pathways, particularly the IL-6R/gp130 cascade and mitochondrial stress responses
2. Precision formulation: Development of combinations optimized for specific production stages, environmental conditions, and disease pressures
3. Bioavailability optimization: Advanced delivery systems ensuring consistent active compound release and absorption
4. Microbiome-host interaction mapping: High-resolution characterization of microbial community shifts and their functional consequences
5. Economic validation: Large-scale production trials assessing cost-effectiveness compared to AGPs and disease management costs

Conclusions

The scientific evidence demonstrates that standardized phytomolecules operate through well-characterized biological mechanisms that substantially replicate those of AGPs:

1. Anti-inflammatory effects reducing energetic costs of immune activation
2. Mitochondrial hormesis enhancing energy metabolism and cellular resilience
3. Selective microbiota modulation supporting beneficial bacteria while controlling pathogens
4. Intestinal barrier enhancement improving nutrient absorption and reducing translocation
5. Antioxidant activity mitigating oxidative stress and supporting immune function

When properly standardized and formulated for controlled release, phytomolecules deliver growth promotion, feed efficiency improvements, and disease resistance comparable to AGPs, while potentially offering advantages in AMR risk profile, stress resilience, and consumer acceptance.

The mechanistic convergence between AGPs and phytomolecules, coupled with demonstrated efficacy in controlled trials, provides producers with confidence that science-based phytomolecular interventions represent legitimate alternatives to AGPs. Success depends on product standardization, appropriate dosing, and understanding that phytomolecules work through fundamental biological pathways rather than undefined or mystical mechanisms.

As the livestock industry continues to navigate the post-AGP era, standardized phytomolecules offer a scientifically sound, mechanistically validated approach to maintaining animal performance, health, and welfare while addressing antimicrobial resistance concerns.

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By Dr. Inge Heinzl, Editor, and Predrag Persak, Regional Technical Manager North Europe

For profitable pig production, efficient energy metabolism is essential. Every kilojoule consumed must be wisely spent – on maintenance, growth, reproduction, or defense. An impacted energy metabolism due to disease or stress impacts animal performance and farm profitability.

Different faces of energy

Energy metabolism determines how efficiently pigs convert feed into body mass. The Gross energy (GE) of the diet, which the use of a calorimeter can determine, is progressively reduced by losses in feces (→digestible energy – DE), urine, gases (→metabolizable energy – ME), and heat, resulting in the →net energy (NE), which is then available for maintenance and performance (growth, milk…).

The requirements for maintenance include the minimum energy that an organism needs to maintain essential functions under standardized conditions and at complete rest. This includes respiration, thermoregulation, tissue turnover, and immune system activity. Only energy in excess of these needs is available for performance. The ratio between additional retained energy and additional energy intake defines the incremental efficiency of nutrient utilization. Under normal conditions, healthy, fast-growing pigs display high incremental efficiencies for both protein and energy deposition by channeling energy efficiently into lean tissue and approximately 25-30% of the metabolizable energy from the feed is used for maintenance, 20-25% for lean gain, and the rest for fat deposition, driving daily gain and carcass quality (Patience, 2019).

However, disease, immune stress, and suboptimal environmental conditions can disrupt this delicate balance, diverting nutrients from growth to survival processes (Obled, 2003). The activation of the immune system leads to reduced feed efficiency, slower growth, and inferior meat quality.

Disease generates costs

The health challenge of disease causes energy loss through several key mechanisms (Patience, 2019).

1. The activation of the immune system becomes an energetic priority. It consumes significant amounts of energy and nutrients, such as glucose and specific amino acids, to produce immune cells and acute-phase proteins, such as haptoglobin and CRP, and to combat pathogens. The nutrients are redirected away from performance toward immune defense, i.e., less energy available for growth performance or even a mobilization of body reserves (fat deposits). A study conducted by Huntley et al. (2017) showed a 23.6% higher requirement for metabolizable energy to activate and maintain the immune system, resulting in a 26% lower ADG.
2. Physiological responses to disease, such as fever (heat production), shivering, or increased physical activity due to discomfort or listlessness, require energy.
3. Additional lower feed intake due to reduced appetite, leading to less energy consumption and intensifying the problem of energy repartitioning.

Environmental challenges are energy-consuming

Besides environmental conditions that cause disease due to high pathogenic pressure, environmental challenges are often related to thermoregulation.

1. Cold stress

In the case of cold stress, the ambient temperature falls below the pig’s lower critical temperature. The animal must spend extra energy to produce heat and maintain a constant body temperature. Alternatively, it can achieve this through shivering (muscle friction generates heat) and the release of thyroid hormones, which increase the metabolic rate and boost body temperature. Another possibility is huddling with other pigs. If the pigs eat more to gain extra energy for warmth, they increase production costs.

2. Heat stress

Excessive temperature leads to heat stress, and the animals attempt to cope through several mechanisms. Increased respiratory evaporation by panting is energy-intensive. Other possibilities are lying spread out on cool surfaces (conduction), seeking shade, and reducing physical activity to minimize heat production. To reduce metabolic heat production, pigs decrease their feed intake; however, this results in an energy deficit and likely mobilizes body reserves, especially in lactating sows.

3. Poor housing and management

High ventilation rates, draughts, wet floors, high stocking densities, and, too often, mixing of pigs are other stressors that require adequate energy-consuming responses. Also, an environment that facilitates excessive heat loss, e.g., through cold concrete floors, constrains the pigs to expend more ME to compensate. Poor-quality air with high levels of harmful gases, such as ammonia or hydrogen sulfide, or dust can lead to respiratory issues and energy expenditure for immune defense.

What are the detailed consequences?

Energy required for immune defense cannot be used for the production of meat, milk, or eggs. Several energy-consuming processes are triggered during an immunological challenge.

Glucose, an important energy source

Several scientists (Spurlock, 1997; Rigobelo and Ávila, 2011) have stated that glucose is primarily used to meet the increased energy demands of an activated immune system. According to Kvidera et al. (2017), the reason might be that stimulated leucocytes change their metabolism from oxidative phosphorylation to aerobic glycolysis (Palsson-McDermott and O’Neill, 2013). A trial conducted by Kvidera et al. (2017) confirmed the high need for glucose. In their trial with E. coli LPS-challenged crossbred gilts, they measured the amount of glucose required to maintain normal blood glucose levels (euglycemia). They calculated that an acutely and intensely activated immune system requires 1.1 g of glucose/kg body weight0.75/h. As they obtained similar results in ruminants (Kvidera et al., 2016 and 2017), they regard this glucose requirement as conserved across species and physiological states. In a confirming study, McGilvray and coworkers (2018) observed a significant (P<0.01) decrease in blood glucose in pigs after injection of E. coli LPS.

A further energy-consuming process is the increase in body temperature (fever): To increase body temperature by 1°C, the metabolic rate must be raised by 10-12.5% (Evans et al., 2015). 

Influence on protein metabolism

Stimulation of the immune system in growing pigs may lead to a redistribution of amino acids from protein retention to immune defense. Amino acids are needed as a ‘substrate’ to synthesize immune system metabolites, such as acute-phase proteins (e.g., haptoglobin, a-fibrinogen, antitrypsin, lipopolysaccharide-binding protein, C-reactive protein, and others (Rakhshandeh and De Lange, 2011)), immunoglobulins, and glutathione (Reeds and Jahoor, 2001). This impacts the requirements for amino acids quantitatively but also qualitatively, i.e., the amino acid profile. Various studies indicated an increased need for Methionine, cysteine, branched-chain amino acids (BCAAs), aromatic amino acids, Threonine, and Glutamine during immune system stimulation (Reeds et al., 1994; Melchior et al., 2004; Calder et al., 2006; Rakhshandeh and de Lange, 2011; Rakhshandeh et al., 2014).

If the required amino acids are not available, they must be either synthesized or obtained from body protein. This costs energy, leads to muscle mass degradation, and causes an imbalance in amino acid levels. Excess amino acids are catabolized, resulting in an increase in blood urea nitrogen (BUN). McGilvray et al. (2018), e.g., observed a 25% increase in BUN in their study, in which they stimulated pigs’ immune systems with LPS.

Another possibility is using amino acids as energy sources. L-Glutamine, for example, is a crucial energy source for immune cells and the primary energy substrate for mucosal cells (Mantwill, 2025).

Carcass and meat quality

As already mentioned, immune stimulation or disease leads to protein degradation. Plank and Hill (2000) reported a loss of up to 20% of body protein (mainly skeletal muscle) in critically ill humans over 3 weeks. This protein degradation influences carcass yield and quality by reducing the amount of muscle meat.

Another effect is a decrease in the muscle cross-sectional area of fibers and a significant shift from the myosin heavy chain (MHC)-II towards the MHC-I type (Gilvray et al, 2019)

How can feed additives support pigs in health challenges?

Health challenges can occur due to infections by bacteria, viruses, fungi, or protozoa, as well as due to myco-, exo-, or endotoxins. Phytomolecules-based and toxin-binding can help animals cope with these health challenges.

Phytomolecules have several health-supporting effects

Phytomolecules can support animals in the case of a health challenge by directly fighting bacteria – antimicrobial effect (Burt, 2004; Rowaiye et al., 2025), scavenging free radicals – antioxidant effect (Saravanan et al., 2025; Dhir, 2022), or mitigating infection – anti-inflammatory effect (Saravanan et al., 2025). 

A trial with the phytomolecules-based product Ventar D demonstrated its antimicrobial and microbiome-modulating effects (Heinzl, 2022). The product clearly reduced the populations of Salmonella enterica, E. coli, and Clostridium perfringens but spared the beneficial lactobacilli.

The anti-inflammatory effects of phytomolecules inhibit the activity of pro-inflammatory cytokines and chemokines from endotoxin-stimulated immune cells and epithelial cells (Lang et al., 2004; Lee et al., 2005; Liu et al., 2020), and there is an indication that the anti-inflammatory effects might be mediated by blocking the NF-κB activation pathway (Lee et al., 2005). A trial confirmed this thesis by showing a dose-dependent reduction of NFκB activity in LPS-stimulated mouse cells (-11% & -54% with 50 & 200 ppm Ventar D, respectively) (Figure 1).

Additionally, Ventar D increases interleukin-10, a cytokine with anti-inflammatory properties, and decreases interleukin-6, a pro-inflammatory cytokine. The result is a dose-dependent decline in the ratio of IL-6 to IL-10 (Figure 2), indicating the effectiveness of the product.

The effects of Ventar D, which support the immune system and redirect energy to enhance growth performance, result in higher daily gains and improved feed conversion. This was observed in a trial conducted on a commercial farm in Germany, using, on average, 26-day-old weaned piglets with a mean body weight of approximately 8 kg. Just after weaning, young animals experience stress (new feed, new groups, and separation from the dam) and are more susceptible to disease.

Two groups of piglets were fed either the regular feed of the farm (Control) or the regular feed + 100 g Ventar per MT of feed. The results for final weight and FCR are shown in Figures 3 and 4

Toxin-binding products support animals against health challenges caused by toxins

As mentioned, various toxins, including myco-, endo-, and exotoxins, can harm animals. The danger of mycotoxins lurks in many feeds, and exo- and endotoxins derive from bacteria. Toxin-binding products, possibly supplemented with phytomolecules that support health (e.g., liver protection), can help animals cope with these challenges.

Solis Max 2.0, a toxin solution containing bentonite and phytomolecules, showed excellent binding performance for myco- and endotoxins (Figures 5 and 6).

Trial with endotoxins

Two samples were prepared: one with only 25 EU (1 EU equivalent to approximately 100 pg or 10,000 cells) of LPS of E. coli O55:B5 LPS/mL solution, and one with the same concentration of LPS but also containing 700 mg Solis Max 2.0/mL.

Solis Max 2.0 bound about 80% of endotoxin.

Trial with mycotoxins

In another in vitro trial, the binding capacity of Solis Max 2.0 for six different kinds of mycotoxins was evaluated. For that purpose, samples with 800 ppb AFB1, 400 ppb OTA, 800 ppb DON, 300 ppb T2, 2,000 ppb FB1, or 1,200 ppb ZEN were prepared, and Solis max was added at two inclusion rates, one corresponding to 1 kg/t, the other to 2 kg/t. The binding capacities ranged from 40.7% for OTA to 96% for AFB1, with the lower inclusion rate, and from 61.5% for OTA to 99% for AFB1, with the higher inclusion rate.

Health support by toxin-binding solutions improves performance

The mitigating effects of Solis Max concerning the negative impact of toxins are also reflected in performance. A trial involving 24 female weaned piglets was conducted to evaluate the mitigating effects of Solis Max in the event of a challenge with a naturally contaminated diet (3,400 ppb of DON and 700 ppb of ZEA). Solis Max was added to one half of the challenged piglets. The addition of Solis Max to the contaminated diet not only compensates for growth performance parameters, such as weight gain and feed conversion, but also for Vulva and tail necrosis scores. The results are shown in Figures 7-11.

Tools are available to prevent the unnecessary expenditure of energy for immune protection

As the various references in the article demonstrate, health challenges such as pathogens or toxins not only spoil the appetite of animals but also require energy due to the activation of the immune system. Products based on phytomolecules, as well as toxin solutions, can help animals cope with these challenges and conserve energy for improved performance.

References:

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Recently, The Poultry Site’s Sarah Mikesell interviewed Predrag Persak, EW Nutrition’s Regional Technical Manager for Northern Europe. The conversation covered topics as wide as sustainability and challenges in poultry production, and as narrow as intestinal permeability. Thanks to The Poultry Site for the great talk!

Watch the video

Sarah Mikesell, The Poultry Site: Hi, this is Sarah Mikesell with The Poultry Site, and today we are here with Predrag Peršak. He is the Regional Technical Manager for Northern Europe with EW Nutrition. Thanks for being with us today, Predrag.

Predrag Peršak, EW Nutrition: Nice to be here, Sarah. Thank you for inviting me.

SM: Very good. It’s nice to visit with you. And today, Predrag and I are in Berlin, Germany, at an exclusive event for the poultry industry called Producing for the Future, which is sponsored by EW Nutrition. You are one of our speakers today, Predrag, so I’m going to ask you just a few questions to let everybody know a little bit about your presentation.

You’ve described animal nutrition as “never boring and never finished.” What makes this field so dynamic and constantly evolving for you?

PP: I’ve been in animal nutrition for about 25 years. And in those 25 years, I would say that not even half a year passed without something extraordinary happening. From genetics to animal husbandry, especially here in Europe, we also have a lot of pressure from consumers and slaughterhouses to adapt production to the needs of the customers.

Sustainability, sourcing raw materials, and the variety of raw materials available in Europe – and the constant development of new ones – make life for an animal nutritionist very, very interesting. It’s also very challenging, and through these challenges you learn a lot.

So, applying what we learned 20 years ago is simply not enough anymore. For someone who wants to be challenged every day with new things, this is definitely the right industry to be in – especially now.

SM: Excellent. Can you explain your holistic approach to animal nutrition and how considering multiple factors benefits practical applications on farms?

PP: The concept of a holistic approach in animal nutrition is not new. But for me – being both a veterinarian and a nutritionist – it means having deeper insight into the animal itself, into all the metabolic processes, and also into the external influences: husbandry, genetics, diseases, and management. Looking at how all of these interact, we can only really solve problems by looking at the animal as a whole system.

The same applies to feed production. You cannot look at a feed mill as just one compartment. You have to look at sourcing raw materials, their quality, how they are processed – milling, pelleting, and other technologies – and then see how that feed performs on the farm.

So, a holistic approach can be applied both from the animal perspective and from the feed production perspective, across all steps and processes. This is something we use and promote daily in our work with customers.

SM: Very good. You’ve worked with unconventional protein and fiber sources. We’re hearing a lot more about that recently. What are those, and what potential do they bring to animal nutrition?

PP: When I talk about unconventional protein and fiber sources, we need to remember that the global feed production scene is very diverse. What applies in the U.S. or Brazil does not necessarily apply in Europe or the Far East.

Here in Europe, we try to use not by-products but co-products of food production. For example, different fractions of rapeseed or sunflower meal, which are widely produced in Europe but not often used by mainstream nutritionists due to certain limitations. By finding the right processing methods and combining them with technologies, we can make these unconventional materials usable in mainstream nutrition.

The same goes for fiber sources. Both fermentable and structural fibers are increasingly important for intestinal and digestive development, as well as for overall animal health. So, processing fibers in ways that maximize usability while minimizing negative effects is a big part of my work.

SM: From a cost standpoint for producers, are those lower-cost inputs, or just alternatives they need to look at?

PP: In Germany we have a perfect expression for this: “yes and no.” There is always pressure on price, especially in poultry, because food must be accessible to everyone. But at the same time, food must not harm the environment or human health, and we should use all resources not fit for humans but still usable for animals.

So, it’s not only about cost – about availability and sustainability. Working with just two, three, or five raw materials for a long time is not the way forward. The way forward is to think of everything that can be used properly, for the benefit of the animals, and ultimately to produce enough food for the world.

Also, using locally available products is important. Feed production is very diverse around the world—raw materials in Southeast Asia differ completely from those in Europe, Brazil, or the U.S. Using technologies to enable the use of locally produced by-products makes production not only sustainable, but also economically viable for local communities. That’s really the core of the feed industry: using what is produced locally.

SM: Interesting. Very cool. How does your interdisciplinary work across poultry, pigs, and ruminants give you unique insights that might be missed with a narrower focus?

PP: I come from a small feed mill in a small country, Croatia. There, you don’t have deep specialization by species or even by category, as you find in larger markets. Specialization has its advantages, but it can also limit creativity and “outside-the-box” thinking.

By working with ruminants, I learned about fermentation processes – knowledge that can be applied to pigs and even to poultry. For example, fermentation can reduce anti-nutritional factors, allowing higher inclusion levels of certain raw materials in poultry diets.

With pigs, fermentation of fibers – especially in piglets – is crucial, and some of that knowledge could be applied to turkeys, where we still face health issues.

So, working across species demands a lot – it leaves little time for other things – but it opens up unique perspectives and cross-species applications that benefit the entire livestock industry.

SM: I was talking with someone yesterday about mycotoxins – there’s a lot of research in pigs but less in poultry. That’s kind of what you’re talking about, right? Applying knowledge across species?

PP: Absolutely. We’re focused now on poultry, but we can learn from poultry too – not only about feeding but also about farm management, biosecurity, and more. These lessons can also apply to pigs or ruminants.

It’s all holistic – you cannot solve everything with nutrition alone. It’s always a package.

SM: You presented today about the importance of intestinal permeability. Why is it important, and how can understanding it impact animal health and performance outcomes?

PP: Intestinal permeability is one of the key features we use to describe gut health. Personally, I’m very practical. For 20 years we’ve talked about “gut health,” but the real question for veterinarians and nutritionists is: what do we actually do with that knowledge?

In my presentation, I explained intestinal permeability as a “point of no return” in gut health. When leaky gut develops, everything else can deteriorate – faster or slower – but it won’t return to normal without intervention.

By comparing how different stressors or pathogens impact intestinal permeability, we can better understand severity and decide where to focus. Nutritionists already pay attention to thousands of factors, but we need to identify the most impactful ones. That was my key message: focus on the most important drivers.

SM: And leaky gut has really become something the whole industry is talking about, right? I’ve even seen it in human health – my doctor has posters about it.

PP: Exactly. Across cows, pigs, and poultry, leaky gut is getting a lot of attention. It’s a physiological or pathophysiological feature that marks the point of no return.

We can talk about dysbiosis and all the causes, but once you reach leaky gut, you understand where intervention is needed. And it’s not just hype. For example, recently Nature published research showing certain types of human bone marrow conditions are linked to leaky gut and microbial influence on blood processes.

So, this is not a passing trend. It’s fundamental. And once we solve one issue, another door opens. That’s why this industry is never boring.

SM: Very good. Well, thank you for all the information today, Predrag.

PP: Thank you, Sarah. It was a pleasure to talk with you.

Watch the video on The Poultry Site.

Author: Ajay Bhoyar, Sr. Global Technical Manager, EW Nutrition

As the poultry industry seeks economical and nutritious feed ingredients, distillers’ dried grains with solubles (DDGS), a co-product of grain-based ethanol production, presents a valuable option providing beneficial protein, energy, water-soluble vitamins, xanthophylls, and linoleic acid. However, the inherent variability in DDGS nutrient composition and high fiber content can pose challenges for consistent inclusion in poultry feeds. The strategic use of feed enzymes has become a significant area of focus to overcome these limitations and further enhance the nutritional value of DDGS in poultry diets. This article will explore the optimization of DDGS utilization in poultry feeds by emphasizing the inclusion of xylanase enzyme that can efficiently degrade the insoluble arabinoxylans. By understanding the factors affecting DDGS quality and strategically employing xylanase, poultry producers can potentially achieve higher inclusion rates of this readily available byproduct, aiming to reduce feed costs while maintaining or even improving production performance and overall health.

Price competitiveness of DDGS

The price of DDGS relative to other feed ingredients, primarily corn and soybean meal, is a significant factor in its global utilization. DDGS often partially replaces these traditional energy (corn) and protein (soybean meal) sources in animal feeds, leading to significant diet cost savings for poultry producers. DDGS contains a high amount of a combination of energy, amino acids, and phosphorus. However, it is usually undervalued as its price is mainly determined based on the prevailing prices of corn and soybean meal.

Variability in the nutritional quality of DDGS

The nutrient composition of DDGS varies based on the starting grain, ethanol production methods, and drying processes. Generally, DDGS contains high levels of protein, fiber, and minerals, with varying amounts of fat and starch depending on the type of grain used and how it is processed. DDGS has a reputation for having variable nutrient composition, protein quality, and a high content of mycotoxins (Stein et al., 2006; Pedersen et al., 2007; Anderson et al., 2012). High quantities of DDGS in feed increase dietary fiber, adversely affecting nutrient digestibility.

The variations in production methods lead to significant differences in the following nutritional components of DDGS:

Crude Fat: This is one of the most variable components, ranging from 5 to 9 percent in reduced-oil DDGS and greater than 10 percent in traditional high-oil DDGS.

Energy: The apparent metabolizable energy (AMEn) for poultry varies among DDGS sources. Fiber digestibility and the digestibility of the extracted oil also contribute to this variability. The high temperatures during the drying stage of DDGS production accelerate lipid peroxidation, forming breakdown products from the fats. This peroxidation contributes to the changes and variability observed in the fat component of DDGS and is a factor that can affect nutrient digestibility and overall energy value.

Crude Protein and Amino Acids (especially Lysine): While crude protein content might not always increase inversely with fat reduction, the digestibility of amino acids, especially lysine, can be affected by drying temperatures. Lysine digestibility of DDGS is a primary concern of poultry nutritionists due to the susceptibility of this amino acid to Maillard reactions during the drying process of DDGS, which can reduce both the concentration and digestibility of lysine (Almeida et al. 2013). Prediction equations have been developed to accurately estimate actual AMEn and standardized ileal digestible amino acid content of DDGS sources based on chemical composition.

Phosphorus: The phosphorus content can vary depending on the amount of Condensed Distiller’s Solubles (CDS) added. The bioavailability of phosphorus can also be influenced by processing. The phosphorus content in the corn DDGS may vary from 0.69 to 0.98 % (Olukosi and Adebiyi, 2013).

Fiber: The neutral detergent fiber (NDF) content is another variable component. Differences in processing conditions among ethanol plants can lead to variations in fiber digestibility.

Table 1. Variation in composition of corn DDGS sources (dry matter basis; adapted from (Pederson et al., 2014)

Nonstarch Polysaccharides (NSP) in DDGS

Non-starch polysaccharides (NSP) are a significant component of DDGS. The NSP profile of DDGS is crucial for understanding its digestibility and energy content.​ The corn DDGS has a complex fiber structure that may limit its digestibility in swine and poultry. NSPs in corn DDGS represent 25-34% of its composition, primarily insoluble (Pedersen et al. 2014). The complexity of the fiber structure in corn DDGS makes it more challenging to degrade with enzymes than wheat DDGS. Therefore, while including DDGS in the poultry feeds, choosing an exogenous xylanase enzyme that is highly efficient in breaking down both soluble and insoluble arabinoxylans is essential for maximum energy utilization.

Use of xylanase in DDGS diets for poultry

Supplementing exogenous enzymes in swine and poultry diets have numerous potential benefits including: reduction of digesta viscosity to enhance lipid and protein digestion; increase the metabolizable energy content of the diet; increase feed intake, growth rate and feed conversion; decreased size and alter the microbial population of the gastrointestinal tract; reduce water consumption and water content of excreta in poultry; reduce the amount of excreta as well as ammonia, nitrogen and phosphorus content (Khattak et al., 2006). The selection of a specific enzyme must be based on the type and availability of the target substrate in the diet.

The improved energy utilization of DDGS in poultry can be achieved through the enzymatic degradation of fiber (NSP). Nonstarch polysaccharides within DDGS exist in matrices with starch and protein, so NSP degradation via exogenous enzymes can also release other nutrients for subsequent digestion and absorption (Jha et al. 2015).

The cell wall matrix in corn DDGS is more complex. Moreover, the most readily degradable arabinoxylan for the fiber-degrading enzymes is modified during DDGS production (Pedersen et al. 2014). Many studies reported a greater branch density and complexity of corn arabinoxylan than wheat (Bedford, 1995; Saulnier et al.,1995a; Jilek and Bunzel, 2013; Yang et al., 2013). These observations indicate that the fiber-degrading enzymes applied for the degradation of corn DDGS need to be targeted towards highly complex substrates. This calls for selecting xylanase, which effectively breaks down the insoluble arabinoxylans in diets.

Axxess XY: Highly effective xylanase in breaking down soluble and insoluble arabinoxylans

A bacterial GH10 family xylanase, like Axxess XY, is more beneficial in animal production due to their efficient mechanism of action, broader substrate specificity, and better thermostability. Generally, the GH10 xylanases exhibit broader substrate specificity and can efficiently hydrolyze various forms of xylan, including soluble and insoluble substrates. GH10 xylanases exhibit higher catalytic versatility and can catalyze the cleavage of the xylan backbone at the non-reducing side of substituted xylose residues, whereas GH11 enzymes require unsubstituted regions of the xylan backbone (Collins et al., 2005; Chakdar et al., 2016).

Fig.1. Activity of a bacterial GH10 xylanase against soluble and insoluble arabinoxylans

Axxess XY facilitates DDGS use and reduces the cost of broiler production.

Including xylanase enzyme, which is highly effective in breaking down soluble and insoluble arabinoxylans in poultry feeds, can reduce feed costs, allowing higher inclusion of DDGS while maintaining the bird’s commercial performance.

In a recently conducted 42-day trial at a commercial farm, Axxess XY maintained broiler performance with a 100 kcal/kg reduction in metabolizable energy and 8% use of Corn DDGS in a corn-SBM based diet (Figure 2). This significantly reduced feed cost/kg body weight.

Incorporating DDGS into poultry diets presents a sustainable and cost-effective solution, but its full potential is often limited by variability in nutrient composition and high fiber content. Xylanase enzymes, particularly those in the GH10 family like Axxess XY, can overcome these barriers by breaking down complex arabinoxylans and unlocking inaccessible nutrients. With proven benefits in energy utilization, nutrient digestibility, and overall production efficiency, xylanase inclusion emerges as a strategic approach to optimize DDGS usage, ultimately supporting economic and environmental sustainability goals in poultry production.

References

Almeida, F.N.; Htoo, J.K.; Thomson, J.; Stein, H.H. Amino acid digestibility of heat-damaged distillers’ dried grains with soluble fed to pigs. J. Anim. Sci. Biotechnol. 2013, 4, 2–11.

Bedford, M.R., 1995. Mechanism of action and potential environmental benefits from the use of feed enzymes. Anim. Feed Sci. Technol. 53, 145–155.

Chakdar, Hillol, Murugan Kumar, Kuppusamy Pandiyan, Arjun Singh, Karthikeyan Nanjappan, Prem Lal Kashyap, and Alok Kumar Srivastava. “Bacterial Xylanases: Biology to Biotechnology.” 3 Biotech 6, no. 2 (June 30, 2016). https://doi.org/10.1007/s13205-016-0457-z.

Collins, Tony, Charles Gerday, and Georges Feller. “Xylanases, Xylanase Families and Extremophilic Xylanases.” FEMS Microbiology Reviews 29, no. 1 (January 2005): 3–23. https://doi.org/10.1016/j.femsre.2004.06.005.

Jha, R.; Woyengo, T.A.; Li, J.; Bedford, M.R.; Vasanthan, T.; Zijlstra, R.T. Enzymes enhance degradation of the fiber–starch–protein matrix of distillers dried grains with solubles as revealed by a porcine in vitro fermentation model and microscopy. J. Anim. Sci. 2015, 93, 1039–1051.

Jilek, M.L., Bunzel, M., 2013. Dehydrotriferulic and dehydrodiferulic acid profiles of cereal and pseudocereal flours. Cereal Chem. J. 90, 507–514

Jones, C.K., Bergstrom, J.R., Tokach, M.D., DeRouchey, J.M., Goodband, R.D., Nelssen, J.L., Dritz, S.S., 2010. Efficacy of commercial enzymes in diets containing various concentrations and sources of dried distillers’ grains with solubles for nursery pigs. J. Anim. Sci. 88, 2084–2091.

Khattak, F.M., T.N. Pasha, Z. Hayat, and A. Mahmud. 2006. Enzymes in poultry nutrition. J. Anim. Pl. Sci. 16:1-7.

Olukosi, O.A., and A.O. Adebiyi. 2013. Chemical composition and prediction of amino acid content of maize- and wheat-distillers’ Dried Grains with Soluble. Anim. Feed Sci. Technol. 185:182-189.

Pedersen M. B., Dalsgaard S., Bach Knudsen K.E., Yu S., Lærke H.N., Compositional profile and variation of Distillers Dried Grains with Solubles from various origins with focus on non-starch polysaccharides, Animal Feed Science and Technology, Volume 197, 2014, Pages 130–14.

Saulnier, L., Vigouroux, J., Thibault, J.-F., 1995a. Isolation and partial characterization of feruloylated oligosaccharides from maize bran. Carbohydr. Res. 272,241–253.

Yang, J., Maldonado-Gómez, M.X., Hutkins, R.W., Rose, D.J., 2013. Production and in vitro fermentation of soluble, non-digestible, feruloylated oligo- andpolysaccharides from maize and wheat brans. J. Agric. Food Chem.

Yoon, S.Y., Yang, Y.X., Shinde, P.L., Choi, J.Y., Kim, J.S., Kim, Y.W., Yun, K., Jo, J.K., Lee, J.H., Ohh, S.J., Kwon, I.K., Chae, B.J., 2010. Effects of mannanase and distillers’ dried grain with solubles on growth performance, nutrient digestibility, and carcass characteristics of grower-finisher pigs. J. Anim. Sci. 88,181–191.

Conference Report

Achieving high performance and superior meat quality with preferably low investment – and here, we speak about feed costs, which account for up to 70% of the total costs – is a considerable challenge for pig producers. The following will focus on the effects of genetic enhancements and nutrient quality on overall pig performance.

Effect of body weight and gender on protein deposition

Based on Schothorst Feed Research recommendations for tailoring nutritional strategies to enhance feed efficiency and overall productivity, the following facts must be considered:

• Castrates, boars, and gilts have significantly different nutritional requirements due to variations in growth rates, body composition, and hormonal influences. For instance, testosterone significantly impacts muscle development and protein metabolism, increasing muscle mass in males. In contrast, ovarian hormones may inhibit muscle protein synthesis in females, contributing to differences in overall protein deposition. Boars, therefore, require higher protein levels to support muscle growth. Castrates typically have a higher FCR compared to gilts and boars due to higher feed intake. Split-sex feeding allows for diet adjustments to optimize growth rates and reduce feed costs per kilogram gained.
• Different body weight ranges: because puberty is delayed in modern genetics, we can produce heavier pigs without compromising carcass quality. Given that a finisher pig with 80-120 kg bodyweight consumes about half of the total feed of that pig, Dr. Fledderus concluded that extra profit could be realized with an extra feed phase diet for heavy pigs. Implementing multiple finisher diets can help reduce feed costs by allowing for lower nutrient concentrations, such as reducing the net energy and standardized ileal digestible lysine in later phases, without compromising performance.

Decision-making according to feedstuff prices

Least cost formulation is commonly used by nutritionists to formulate feeds for the lowest costs possible while meeting all nutrient requirements and feedstuff restrictions at the actual market prices of feedstuffs. However, diet optimization is more complex. The real question is, “How do you formulate diets for the lowest cost per kilogram of body weight gain?” You must always consider your specific situation, as economic results vary greatly and depend mainly on the prices of pork and feed and pig growth performance (e.g., feed efficiency, slaughter weight, and lean percentage).

How can you optimize your feeding strategy? Reducing net energy (NE) value will result in more fiber entering the diet. This makes sense if fiber by-products are cheaper than cereals. In contrast, an increase in the NE value will increase the inclusion of high-quality proteins and synthetic amino acids. It will use more energy from fat and less from carbohydrates.

The effects of diet composition on meat quality and fat composition also need to be considered.

How can nutrition improve meat quality?

Nutritional strategies not only improve the sensory attributes of pork but also enhance its shelf life, ultimately leading to higher consumer satisfaction and better marketability. Some of the factors Dr Fledderus considered included:

Improving fat quality

The source of dietary fat significantly impacts the quality of pork fat. Saturated fats tend to produce firmer fat, while unsaturated fats can lead to softer, less stable fat deposits. Diets high in unsaturated fats are more prone to lipid oxidation, negatively affecting shelf life and overall meat quality. The deposition of polyunsaturated fatty acids is only from dietary fat. Saturated fats in pork, partly originates from dietary fat and are also synthesized de novo. So, the amount of polyunsaturated fatty acids in pork depends on the content and composition of dietary fat, which can negatively affect the shelf life and perception of pork meat.

The iodine value (IV) is a measure of the degree of unsaturation in fats. A higher IV indicates a higher proportion of unsaturated fatty acids, leading to softer fat. Pork fat with an IV lower than 70 is considered high quality, as it tends to be firmer and more desirable for processing.

As per the American Oil Chemists Society, IV is calculated as:

IV = [C16:1] × 0.95 + [C18:1] × 0.86 + [C18:2] × 1.732 + [C18:3] × 2.616 + [C20:1] × 0.785 + [C22:1] × 0.723

(brackets indicate concentration (%) of C16:1 palmitoleic acid, C18:1 oleic acid, C18:2 linoleic acid, C18:3-linoleic acid, C20:1 eicosenoic acid, C22:1 erucic acid per crude fat)

Implications

Dr. Fledderus concluded that the pigs’ nutritional requirements are dynamic and influenced by factors such as required meat and fat quality, heat stress, slaughter weight, and genetic developments. Tailoring diets based on gender and body weight is crucial for optimizing protein deposition. Accurate information is essential to formulate diets that achieve optimum economic results, not just the least cost.

Continuous monitoring of feedstuff prices and nutritional content allows for timely adjustments in diet formulations, ensuring that producers capitalize on cost-effective ingredients while maintaining nutritional quality.

EW Nutrition’s Swine Academy took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Jan Fledderus, Product Manager and Consultant at the S&C team at Schothorst Feed Research, with a strong focus on continuously improving the price/quality ratio of the diets for a competitive pig sector and one of the founders of the Advanced Feed Package, was a reputable guest speaker in these events.

Conference Report

During the recent EW Nutrition Swine Academies in Ho Chi Minh City and Bangkok, Dr. Jan Fledderus, Product Manager and Consultant at Schothorst Feed Research, discussed that much money is involved in a correct energy evaluation system. “Net energy is 70% of feed costs, and feed is about 70% of total costs.” Therefore, an accurate energy evaluation system is important as it will give:

• Flexibility to use different raw materials
• Reduction of formulation costs
• Best prediction of pig performance
• Match the available dietary energy requirement of the feed to the pig’s requirement

Energy evaluation systems for pigs

The energy value of a raw material or complete feed can be expressed using different energy evaluation systems. Net energy (NE) in pigs refers to the amount of energy available for maintenance and production after accounting for energy losses during digestion, metabolism, and heat production. It is a crucial concept in swine nutrition as it provides a more accurate measure of the energy value of feed ingredients compared to other systems like digestible energy (DE) and metabolizable energy (ME). Diets formulated using NE are lower in crude protein than those using DE or ME because the heat lost during catabolism and excretion of excess nitrogen is considered in the NE system.

Effect of energy

Energy is derived from three nutrients: lipids (fats and oils), carbohydrates, and proteins. Using NE values instead of DE or ME values can lead to changes in ingredient ranking when formulating diets. For example:

• Ingredients high in fat or starch may be undervalued in DE systems but receive appropriate recognition in NE evaluations.
• Conversely, protein-rich or fibrous ingredients may be favored in DE systems.

Table 1: Energy values (kcal/kg) of nutrients

Calculation of net energy

Net energy (kcal/kg dry matter) is calculated as:
= 2,577 x digestible crude protein
+ 8,615 x digestible crude fat
+ 3,269 x ileal digestible starch
+ 2,959 x ileal digestible sugars
+ 2,291x fermentable carbohydrates

Factors affecting nutrient digestibility

This raises the obvious question, ‘What is the nutrient digestibility of your raw materials?’ Dr. Fledderus considered several factors that affect nutrient digestibility and, therefore, NE values, including

• Age: as pigs grow, their digestive systems mature, leading to improved nutrient digestibility. Younger pigs typically have lower digestibility rates due to an underdeveloped gastrointestinal tract. Older pigs typically exhibit higher digestibility, especially for fibrous diets, as their digestive systems become more efficient at breaking down complex nutrients.
• Physiological stage: the digestibility of diets can vary between pregnant and lactating sows. Digestibility is generally higher for gestating sows; lactating sows may have slightly lower digestibility due to higher feed intake. Also, lactating sows do not consume enough feed to meet their energy needs, leading to body tissue mobilization and weight loss.
• Feed intake and number of meals per day: Increased feed intake and more frequent meals can enhance nutrient digestibility. Regular feeding helps maintain gut motility and reduces the risk of digestive disturbances. Studies indicate that pigs fed multiple smaller meals exhibit better nutrient absorption than those fed larger meals less frequently.
• Use of antibiotics and feed additives: including exogenous enzymes and other additives can improve nutrient breakdown and overall digestibility of complex feed components, further influencing ingredient rankings within different energy evaluation systems. Antibiotics can lead to dysbiosis, negatively impacting overall gut health and digestion.
• Feed processing: gelatinized starch is more easily broken down by digestive enzymes, resulting in higher and faster digestibility compared to raw or unprocessed starch. This increased digestibility leads to a greater proportion of energy being absorbed in the small intestine, contributing positively to the NE value of the feed. As the particle size of feed ingredients decreases, the NE increases. While smaller particles generally improve digestibility, excessively fine grinding can lead to adverse effects such as increased risk of gastric ulcers in pigs.
• Intestinal health: a healthy gut is crucial for optimal nutrient absorption. Factors such as the presence of beneficial microbiota and the integrity of the intestinal barrier play significant roles in nutrient digestibility. Conditions like inflammation or dysbiosis can impair nutrient absorption and decrease overall performance.

NE system shows better the “true” energy of the diet

Dr. Fledderus concluded that the NE system offers a closer estimate of pigs’ “true” energy available for maintenance and production (growth, lactation, etc.). This leads to better ingredient rankings, reduced crude protein levels, which decreases nitrogen excretion, and enhanced nutrient utilization, contributing to more sustainable pig production practices. This aligns with increasing demands for environmentally responsible farming methods.

EW Nutrition’s Swine Academy took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Jan Fledderus, Product Manager and Consultant at the S&C team at Schothorst Feed Research, one of the founders of the Advanced Feed Package and with a strong focus on continuously improving the price/quality ratio of the diets for a competitive pig sector, was a reputable guest speaker in these events.

Conference Report

“A good start is half the battle” can be said if we talk about piglet rearing. For this promising start, piglets must eat solid feed as soon as possible to be prepared for weaning. Dr. Jan Fledderus, Product Manager and Consultant at the S&C team at Schothorst Feed Research, shows some nutritional measures that can be taken to keep piglets healthy and facilitate the critical phase of weaning.

Higher number of low-birth-weight pigs in larger litters

Litter size affects piglet quality. Larger litter sizes from hyperprolific sows often result in higher within-litter variation in birth weights. This variability can lead to a higher proportion of low-birth-weight piglets, which are more susceptible to health issues and have lower survival rates. Additionally, low birthweight pigs have an increased risk of mortality, and an improvement in birth weight from 1kg to 1.8 kg can result in 10 kg more body weight at slaughter.

Implementing management practices for low-birth-weight pigs, such as split suckling, can significantly enhance nutrient intake, support immune function, and ultimately contribute to better survival rates and overall health for these vulnerable piglets.

Weaning age determines intake of creep feed

Pigs that consume creep feed before weaning restart faster to eat, have a higher feed intake, and less diarrhea after weaning. For instance, in a field trial, pigs that consumed feed 10 days before weaning had a 62% incidence of diarrhea, whereas in pigs that consumed feed only 3 days pre-weaning, diarrhea incidence increased to 86%.

“As age is the most critical factor for a high percentage of pigs eating before weaning, there is a trend in the EU to increase the weaning age, where some farmers go to 35 days,” remarked Dr. Fledderus.

Furthermore, weaning age is positively correlated with weaning weight. Every day older at weaning improves post-weaning performance and reduces health problems.

Feed management

Creep feed for 7-10 days pre-weaning is essential, not to increase total feed intake, but to train the piglet to eat solid feed to avoid the ‘post-weaning dip.’ After about 15 days of age, piglets can consume more than is provided by milk alone. Dr. Fledderus strongly recommended creep feeding for at least one week before weaning. “Consuming feed before weaning will result in fewer problems with post-weaning diarrhea,” he said.

In addition to creep feeding, a transition diet, from 7 days pre- and 7 days post-weaning, is advised. The composition or form of the transition diet should not be changed.

The key objective of post-weaning diets is to achieve a pH of 2-3.5 in the distal stomach. Pepsin, the primary enzyme responsible for protein digestion, is activated at a pH of around 2.0. Its activity declines significantly at a pH above 3.5, which can lead to poor protein digestion and nutrient absorption.

Fiber as a functional ingredient

Fiber was previously considered a nutritional burden or diluent, but now it is regarded as a functional ingredient. Including dietary fiber, mainly inert fiber such as rice or wheat brans, can increase the retention time of the digesta in the stomach. This extended retention allows for more prolonged contact between digestive enzymes and nutrients, facilitating improved digestion and absorption of proteins and other nutrients. Not only is pH reduced, but because more proteins are hydrolyzed to peptides, there is less undigested protein as a substrate for the growth of pathogenic bacteria and the production of toxic metabolites in the hindgut.

“Size of fiber particles also matters,” said Dr. Fledderus. Coarse wheat bran particles (1,088 μm) have been shown to be more effective than finer particles (445 μm) in reducing E. coli levels in the gut. The larger particle size helps prevent E. coli from binding to the intestinal epithelium, allowing these bacteria to be excreted rather than colonizing the gut.

The understanding of dietary fiber’s role in pig nutrition has evolved, with recent findings indicating that fiber can actually increase feed intake in piglets, contrary to earlier beliefs that it might decrease intake. High-fiber diets often increase feed intake as pigs compensate for lower energy density. This can help maintain growth rates when formulated correctly.

EW Nutrition’s Swine Academy took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Jan Fledderus, Product Manager and Consultant at the S&C team at Schothorst Feed Research, one of the founders of the Advanced Feed Package and with a strong focus on continuously improving the price/quality ratio of the diets for a competitive pig sector, was a reputable guest speaker in these events.