Conference report

Many factors affect eggshell quality, such as nutrition, disease, genetics, environmental conditions, age of birds, stress, egg collection and handling, and packaging and transport. Eggshell quality, however, is primarily related to management and nutrition, not genetics or other factors. It is becoming a bigger issue as the length of the laying period has extended because, as hens get older, shell quality drops.

“The information in the genetics companies’ management guides is for direction and information only, as each egg producer’s production goals and conditions can vary”, says Vitor Arantes, Global Technical Services Manager and Global Nutritionist, Hy-Line International. He advises listening to your birds. For example, “diets should be aligned with the bird’s bodyweight development, rather than the age of birds and following feeding phases according to pre-planned timings for feed changes,” he noted.

Below are some of the nutritional factors impacting eggshell quality that producers should keep top of mind.

Early development and pre-starter diets

“Bodyweight at 6-12 weeks of age is key, but to achieve this goal, bodyweight up to 5 weeks of age is a MUST, stressed,” Dr. Arantes. “This critical period is an investment, so don’t be shy. Poor management in the first 5 weeks will delay production, increase mortality, and prevent the achievement of peak production targets. In turn, it will affect egg quality. Therefore, we must provide proper diets as soon as possible,” he said.

As shown below, chicks hatch with relatively underdeveloped internal organs and systems. During the first 5 weeks of age, the digestive tract and the immune system undergo much of their development. The development of the intestine is crucial for nutrient absorption and will determine a hen’s future production efficiency. Strong intestinal development will also strengthen the immune system and reduce the possibility of future enteric diseases and improve the response to vaccinations.

Multi-phasic body weight development during rearing and the start of lay

Pre-starter diets support the chicks’ transition from being fed by the yolk sac and are relatively high in energy, protein, and the vitamins and minerals required for growth and development. The chicks’ limited digestive capacity post-hatch demands easily digestible raw materials. A crumble containing high-quality, functional ingredients provides a good nutritional start in life. The use of feed additives, such as enzymes to improve digestibility, and synbiotics to aid in the early development of a microbial population and to prevent the intestinal colonization of pathogens, known as competitive exclusion, should be considered.

Teaching hens how to eat – preparing for the pre-peak phase

The objective is to develop sufficient feed intake capacity for the period start of lay, by feeding a developer diet from 10-16 weeks of age. This is a diluted diet with high levels of insoluble fiber to develop feed intake capacity (crop and gizzard).

“You can train pullets to eat by taking advantage of their natural feeding behavior,” commented Dr. Arantes “Because birds consume most of their feed before lights go off, the main feed distribution (60% of the daily ration) should be in the late afternoon, about 2-3 hours before ‘light off’. In the morning, birds will be hungry and finish the feed, including fine particles. Emptying feeders helps to prevent selective eating and will increase the uniformity of the flock. In the middle of the day, there should be no feed in feeders for 60-90 minutes,” he noted.

Don’t neglect the pre-lay phase

Start feeding a pre-lay diet when most pullets show reddening of the combs, which is a sign of sexual maturity. Feed for a maximum of 10–14 days before the point of lay. This is important to increase medullary bone calcium reserves. Large particle calcium should be introduced in this phase. Do not feed pre-lay later than the first egg as it contains insufficient calcium to support egg production.

There can be a negative impact on feed consumption from the sudden increase in dietary calcium levels from 1% to above 4% at the start of lay. Field experience indicates that the use of pre-lay diets helps as a smooth transition between the developer (low calcium and nutrient density) and the peaking diet. Correct feed formulation and matching diet density with consumption will minimize the impact of reduced calcification of bone over the laying cycle and extend the persistency of shell quality. It also helps to avoid the often-reduced appetite/daily feed intake during early production.

The following are suggested for pre-layer feed:

• 1.25 to 1.40% P
• 2.5% Ca (50% coarse limestone)
• 900-1,100g per hen total
• Never before 15 weeks of age
• Never after 2% hen day (HD) egg production

Understand your limestone

Calcium particle size is important for eggshell quality. Fine calcium carbonate particles pass through the gastrointestinal tract in 2-3 hours, whereas particles above 2mm are retained in the gizzard and will slowly solubilize, delaying the calcium assimilation. Eggshell formation takes 12 to 14 hours and occurs mainly during the night period. Providing a high amount of large calcium particle size before the night, when birds are sleeping, will help laying hens to produce a strong eggshell.

The ratio of coarse to fine calcium particles will increase with bird age as below. Changing the particle size ensures that more calcium will be available at night from the diet instead of from the bone.

Calcium particle size recommendations

Particle size	Starter, Grower, Developer	Pre-Lay	Weeks 17-37	Weeks 38-48	Weeks 49-62	Weeks 63+
Fine (<2mm)	100%	50%	40%	35%	30%	25%
Coarse (2-4mm)	–	50%	60%	55%	70%	75%

 

The solubility of limestone may differ according to the source. Calcium with high solubility will not be stored for a long time in the gizzard, negating the particle size effect. Dietary calcium levels may need to be adjusted based on the solubility of your limestone. The in vitro solubility of your limestone source can easily be checked on the farm, with a simple technique using hydrochloric acid. The target is to recover 3-6% of the supplemented limestone.

Water

It’s impossible to have good eggshell quality if you don’t have good water intake and good quality water. For example, excessive salt levels in drinking water can cause persistent damage to shell quality.

Conclusion: invest in the rearing phase

Good nutrition and management practices are key to good shell quality. The rearing period is a key developmental time for future success during the laying period – it is an investment phase.

***

EW Nutrition’s Poultry Academy took place in Jakarta and Manila in early September 2023. Vitor Arantes, Global Technical Services Manager and Global Nutritionist, Hy-Line International, was a distinguished guest speaker in this event.

Dr Daniel Valbuena, Global Manager of Technical Services, Hy-Line International – Conference Report

Heat stress is one of the major environmental stressors in the poultry industry, especially in regions with high temperatures and high humidity. In EW Nutrition’s Poultry Academy in September, the topic was approached in a comprehensive and practical presentation.

A layer’s normal body temperature is about 40° C. Hens are comfortable with an ambient temperature 18° C to 24° C. When that temperature gets above 32° C, the more serious consequences of heat stress occur.

To mitigate the negative effects of heat stress on bird welfare, production, and profitability, essential best practices include those listed below.

Ventilation

Airflow at the birds’ level is key to removing bird heat. Naturally-ventilated barns are particularly at risk of heat stress. Increase the movement of air in open houses with stir fans. Ensure a minimum velocity of 1.8–2 meters/second in the bird areas.

Clean and ensure function of fan louvers. Fan belts should be tightened or changed to avoid slipping or breaking during periods of high temperature. Poorly maintained fans will operate at 50% reduced efficiency.

Air inlets must be adequate to supply the airflow needed to ventilate the house during warm weather. Inadequate inlet space will throttle down the fans and decrease airflow. Inlets should be kept clean and free of anything that might restrict the flow of incoming air. Use baffle boards to direct incoming air onto the birds. Thermostats should be checked for accuracy. An auxiliary power system must be in place in case of a power outage during hot weather.

In addition to running fans throughout the day when it’s hot, fans should run overnight and early morning to bring in cooler air. Air inlets should be adjusted to achieve uniform airflow throughout the building.

In houses equipped with evaporative cooling systems, the pads should be cleaned or replaced when they become clogged. Water flow over the pads should be uniform with no dry areas. Air will flow preferentially through dry areas since there is less resistance. Clean spider webs and dust from window screens frequently to improve ventilation inside the house.

Fans increase air velocity within the house and create a cooling effect.

Foggers

Fogging or misting is effective at low humidity (<60% relative humidity). Excess moisture in the air from using foggers or misters at high humidity can worsen heat stress conditions.

Foggers or misters need to be checked routinely and should run about 2 minutes out of every 10 if the humidity is low, however, run times can be adjusted based on house temperature and humidity. Fogging the inlet air in negative pressure ventilation systems has a good cooling effect.

Fogging systems should have water filters (to keep nozzles from clogging) and have a positive shutoff to prevent dripping causing wet litter.

Roof design and cooling

Insulated roofs (R28 is recommended) reduce the radiation and conduction of solar heat through the roof to the interior of the house. Reflective roofing materials or light-coloured materials are recommended.

Ensure to provide ridge vents at roof level to allow hot air to exit so that fresh air may enter the house through side openings. The roof should have an overhang (minimum 60 cm) to reduce direct and indirect sunlight getting into the house.

Thatching can provide cost-effective insulation, but may need to be replaced every few years, and is difficult to clean, and may harbor vermin.

Use of shade netting (left) or thatching material such as palm fronds, paddy straw, corn stalks, sugarcane tops (right) to reduce solar heating of the roof.

Using roof sprinklers during times of extremely high temperature can remove heat from the roof and cool the inside of the house.

Curtains

Adjustable, porous side-wall curtains can be used control the flow of air into the house, and protect birds from direct, hot winds. Water dripping onto side curtains can reduce the house temperature.

Porous window shades block direct sunlight from entering the house but allow air to pass through.

Bird handling

Management practices that require bird handling, such as beak trimming, transfer, and vaccinations, should be done in the early morning hours, or in the evening, when it’s not so hot. Heat-stressed birds have decreased immune function and may not respond as well to vaccination. Alternatively, if birds are panting, they may breathe in too much of a spray vaccine or ingest too much of a water-administered vaccine. In both situations, birds may exhibit signs of the disease that the vaccines are intended to prevent.

Stocking density

It’s critical not to overstock cages. If stocking density is high, the radiant heat between the birds accumulates and the temperature increases. Birds need to be able to spread their wings to increase airflow around their bodies. Caged birds are more susceptible to heat stress because they are unable to seek a cooler place and there is less opportunity for conductive heat loss in cages. The temperature within a cage can be much higher than the measured air temperature in the walkway. Increased air velocity within the cages increases the convective heat loss and removes trapped air between birds.

Manure management

Manure allowed to accumulate reduces ventilation in cages. Remove manure from the house before the hot season, if practical. Heat produced during the decomposition of manure contributes to the heat load in the house. The presence of large amounts of manure in shallow pit houses or under cage batteries restricts the movement of air.

Be prepared and anticipate

• The key to minimizing the effects of heat stress is to be prepared and anticipate periods of high environmental temperatures, and implementing appropriate management measures prior to the rise in temperatures. Implement cooling systems, such as evaporative cooling pads, misting systems, or fans. Provide natural or artificial shading within the layer house to reduce direct sunlight exposure. Shade structures, curtains, or baffles can help protect the hens from excessive heat.
• Farm personnel should be trained to recognize and respond to heat stress promptly. You should also have an emergency plan in place for extreme heat events.

Dr Daniel Valbuena, Global Manager of Technical Services, Hy-Line International – Conference Report

Feed and water management strategies are essential to help mitigate the negative effects of heat stress on bird welfare, production, and profitability. In EW Nutrition’s Poultry Academy in September, the topic was approached in a comprehensive and practical presentation.

Feed management

Feed consumption of the flock should be closely monitored during hot weather. It is important to rebalance the diet for critical nutrients, particularly amino acids, calcium, sodium and phosphorous according to the birds’ productivity demand (i.e., stage of production) and the observed feed intake. Insufficient amino acid intake is the primary reason for productivity loss during hot weather. Several strategies may be employed to help to manage elevated temperatures and maintain higher levels of feed intake:

• Withdrawing feed from birds 6 hours before peak hot temperatures in the afternoon can lower the risk of heat stress. Encourage as much consumption as possible in the early morning or evening. Using lighting for midnight feeding encourages feed intake.
• One third of the daily feed ration should be given in the morning and two thirds in the late afternoon. An additional advantage is the availability of calcium in the digestive system during shell formation at night and in the early hours of the morning. This will improve shell quality and reduce the birds from depleting bone calcium.
• Normally a maximum 1 hour for feeder clean-out time is recommended, but this can be extended to 3 hours when the temperature exceeds 36°C.
• Consider adding a 1-2-hour midnight feeding.
• Alter feed particle size, either by increasing it or by feeding a crumble diet. With crumble diets in laying flocks, a supplementary source or presentation of large particle limestone is recommended.
• Formulate diets using highly digestible materials, particularly protein sources. Metabolism of excess protein is particularly heat-loading on the bird. Formulate to digestible amino acid targets and do not apply a high crude protein minimum in the formula. Synthetic amino acids can reduce crude protein in the diet without limiting amino acid levels.
• Increasing the proportion of energy contribution from highly digestible lipid, rather than starches or proteins, will reduce the body heat production resulting from digestion. This is known as heat increment and is lowest with the digestion of dietary fat.
• The bird’s metabolizable energy requirement decreases as ambient temperature increases to above 21°C, resulting from a reduction of energy requirements for maintenance. The energy requirement will decrease with the rise of temperature up to 27°C, above which it will start to increase again since the bird needs additional energy for panting to reduce body heat.

Management schedule during times of heat stress

Water management

During periods of high environmental temperature, birds have a high demand for drinking water. The water-to-feed consumption ratio is normally 2:1 at 21°C but increases to 8:1 at 38°C. Adequate drinking water must be available to heat-stressed flocks. Ensure that drinkers have sufficient water flow (>70 mL/minute/nipple drinker). If water flow is less the lines need to be checked for flow restriction. If there’s a build-up of iron and other minerals, it needs to be removed. Don’t forget to routinely check water filters and replace them as needed.

It’s easy to overlook a non-functioning drinker here and there; drinkers must be systematically checked to make sure they’re all working. For floor-reared flocks, providing additional drinkers can help accommodate the increased water consumption.

During hot weather, you need to ensure your water system can accommodate the bird’s increased water consumption, and the additional water demands for foggers, evaporative cooling systems and roof sprinklers. The availability of drinking water to a heat-stressed flock should never be compromised.

Cool water temperatures (<25^oC) will encourage the birds to drink and reduces the birds’ core temperature. Flush water lines and waterers routinely to keep the water fresh and cool, increasing water consumption, and sustaining egg production. If available, ice can also be added to header tanks. When mechanical cooling systems fail, water flushing can serve as an emergency measure during heat stress.

Drinking water from overhead water tanks can become hot if exposed to direct sunlight. These water tanks should be a light color, insulated and covered to avoid direct sunlight. Water tanks are ideally placed inside the house or underground. Water pipes in the house should not be installed close to the roof to avoid heat from the roof warming up the water in the pipes.

Use vitamin (A, D, E and B complex) and electrolyte supplements in the drinking water to replenish the loss of sodium, chloride, potassium, and bicarbonate in the urine. Electrolyte supplements are best used in anticipation of a heat stress period and can be added to drinking water for up to 3 days.

Dr. Vitor Arantes, Global Technical Services Manager and Global Nutritionist, Hy-Line International – Conference Report

The layer industry has gone through significant changes during the past decades and has a remarkable capacity to cope with new challenges. Dr Vitor Arantes, Global Technical Services Manager and Global Nutritionist, Hy-Line International, noted that increased egg production, improved feed efficiency, and adaptation of egg quality and bird welfare to consumer preferences have contributed significantly to the success of the egg industry. However, continuous improvement in egg production per hen housed is the most important selection criteria in layer breeding.

Egg producers needs include:

• More saleable eggs,
• Eggshell quality,
• Easier behaviour
• Housing systems
• Egg size specifics
• Sanitary / environmental challenges
• Profits through productivity

Primary breeders can deliver these producer needs through:

• Having the correct product for each country
• Constant follow up
• Local presence, trust relationship
• Accurate data collection
• Critical data analysis
• Understand the company’s goals
• Customized technical services according to each customer needs

How has genetics changed?

Examples of genetic progress in layers from 1984 to 2022 cited by Dr Arantes include:

• Higher persistency (+30 weeks >90%)
• Higher egg mass (+5.5 kg/hen housed)
• Smaller hen (-21% mature body weight)

Dr Arantes states the record clutch size, defined as the unstopped length of individual egg production on a daily basis, was an amazing 474 days for a White Plymouth Rock hen. This genetic progress necessitates adjustments in nutrition and management.

As shown below, growth and organ development occur at various ages. “There is no margin for mistakes – a lack of growth during a stage could have a detrimental impact on pullet quality and subsequent production,” stressed Dr Arantes.

Multi-phasic growth and development during rearing and start of lay

System	Age (weeks)	Consequence
Gastrointestinal	0-6	Shorter intestinal tract/reduced nutrient absorption
Immune	0-6	Flocks more susceptible to disease challenges
Skeleton	6-12	Shorter frames/less calcium reserves
Muscle	6-12	Impact in persistency of production
Fat	>12	Excess can lead to fatty liver, prone to prolapse and mortality

 0-6 weeks of age

Most of the development of the organs of the digestive tract and the immune system occurs during the first 6 weeks of age. Problems that occur during this period can have negative effects on the function of these systems. Birds stressed during this period may have lifelong difficulties in digesting and absorbing feed nutrients. Immunosuppression may also result from problems during this period, leaving the bird more susceptible to diseases and less responsive to vaccinations.

6-12 weeks of age

Most of the adult structural components – muscles, bones and feathers are obtained during the period of rapid growth that occurs at 6-12 weeks of age. Growth deficiencies during this period will prevent the bird from obtaining sufficient bone and muscle reserves, which are necessary to sustain a high level of egg production and to maintain good eggshell quality. About 95% of the skeleton is developed at the end of the bird’s 13 weeks of life. At this time, the plates of the long bones become calcified and further growth in bone size cannot occur.

12-18 weeks of age

During this period, the growth rate slows, and the reproductive tract matures and prepares for egg production. Muscle development continues and the proliferation of fat cells takes place. Excessive weight gain during this period can result in an excessive amount of abdominal fat. Low body weight and stressful events at this time can delay the start of egg production. From 7-10 days before oviposition of the first egg, the medullary bone that is located within the cavities of the long bones can be increased by feeding the bird a pre-laying ration with higher levels of calcium than the development stage.

Bodyweight is a key factor for flock management as this will influence future performance of birds. Consequently, bodyweight should be controlled during the whole life of the layer flocks. Management, in particular nutrition and lighting programs, can help to control bodyweight so birds can achieve their genetic potential.

Uniformity

Uniformity is the most important KPI in our business. However, with the trend towards larger flocks, maintaining uniformity is becoming more challenging. With larger flocks, it is difficult to source one unique flock which thus usually comprises multiple breeding flocks of different ages. Inevitably, uniformity will be poor, hence the need for tools to address unexpected issues. Lack of uniformity becomes a self-perpetuating cycle – dominant versus dominated.

Many egg producers use average body weights compared to the breeder recommendations as a guide to flock status. However, knowing if you have good body weight uniformity is another valuable management tool. In any flock some birds are lighter or heavier than the average body weight. Poor uniformity makes management decisions, such as lighting, feed amounts or diet phase more difficult.

Ideally, the body weight coefficient of variation (CV) should be +/-10% of the mean, increasing the likelihood that your management decision will be appropriate for most of the flock. Inappropriate diet changes, bird handling, vaccination and transfer can reduce uniformity. Flocks should be at 90% uniformity at the time of transfer to the laying facility. Body weight at point of lay significantly affected egg production and eggshell quality.

Grading into 2 or 3 sub-populations of different average bodyweights may be necessary so that each group can be managed in a way that will achieve good whole flock uniformity at the point of lay. The best predictor of future laying performance is the pullet’s body weight and body type at the point of lay.

Vision egg

Vision Egg is a custom diagnostic tool used to analyze data and emphasize flock performance to achieve the highest genetic potential from Hy-Line layers with recommendations connected to customer profitability. This growing, robust database includes data from over 1 billion hens strengthens our flock performance diagnostic tool for improved profitability for Hy-Line customers.

Hy-Line customers can take advantage of this opportunity by sending flock data to their regional business manager or technical service specialist. The information shared with Hy-Line is kept completely confidential.

Summary

The challenge is not egg numbers, stated Dr. Arantes, but saleable eggs. Correct body weight and high uniformity of the flock at point of lay will result in good performance over the laying period, with high peak production and good persistency of production and the production of good quality eggs. Management is the key factor to regulation of body weight during rearing and at point of lay.

Conference report 

At the recent EW Nutrition Poultry Academy in Jakarta Indonesia, Dr Steve Leeson, Professor Emeritus, University of Guelph, Canada, commented that “genetic progress in layer breeding has been substantial in recent decades. Since 1995, the yearly change has included +1 egg, -0.01 feed/dozen eggs, -10g final bodyweight, 0.02% mortality, and +1 week at >90% egg production. This improved persistency of commercial laying hens enables egg producers to keep flocks longer in production, provided egg shell quality can be maintained.”

He noted that “the increase in hen-housed egg production is mainly due to longer clutch length and improved uniformity of layer flocks. No doubt, there is a trend in cage layers to longer production cycles. A popular commercial goal is 500 eggs in one cycle with no moult, although this has already been surpassed in many flocks. The modern layer is capable of laying 150 eggs per clutch.”

Dr Leeson, however, stressed that “genetic progress and longer laying cycles have consequences. Long laying cycle programmes start during pullet rearing – you can’t make decisions at 72 weeks of age. Instead, you must start with your end goals, such as persistency, egg size and shell quality, in mind. You can then develop a life-cycle approach to feeding, lighting, nutrition, and general management.” Important issues to manage include:

Body weight control – early and late

Mature body weight dictates subsequent egg size. In the past, the common goal was being at, or above, management guide weight recommendations. For extended lay, a larger body weight results in too large an egg past 70 weeks of age, and so it is more difficult to maintain egg shell quality. Now the goal is to grow a slightly smaller pullet, and emphasis changes to achieving adequate early egg size from this smaller bird. This makes pre-lay nutrition for these slightly smaller pullets even more important.

The scheduling of rearing diets is more important than diet formulation. Dr Leeson’s guidelines are:

• Starter diet – 19-20% CP, 2,850-2,900 kcal ME/kg from day old to target pullet body weight
• Grower diet – 17-18% CP, 2,800-2,900 kcal ME/kg from target body weight to mature body size
• Pre-lay diet (or layer diet?) – 16-18% CP, 2,800-2,900 ME/kg, mature body size to first egg

All nutrients are important, but energy is usually limiting for egg number, whereas protein/amino acids influence egg size (and feathering).

There is now even more emphasis on pullet growing to ensure adequate fat reserves through peak production, so birds are in a positive energy balance. The establishment of an energy reserve occurs during the rearing phase and has a significant effect on the bird’s body composition at point of lay.

Egg size control – early and late

The obvious solution to manage body weight (and egg size) is to light-stimulate a smaller pullet, or at least to not light-stimulate a heavy pullet. This achieves a balance between accepting reduced early egg size, versus limiting an increase in egg size late in the production cycle.

Egg size can be increased in smaller early-lay pullets by:

• Reducing environmental temperature, if possible, to stimulate feed intake
• Midnight feeding 19-29 weeks
• Adequate amino acid nutrition intake, tailored to feed intake, especially methionine
• Increased number of feedings/day and increased feed particle size (pellets)

Shell strength is negatively correlated with egg size. To temper egg size late in the cycle, Dr Leeson recommended:

• Body weight control
• Controlled day length: longer day length = increased feed intake, 14 hours maximum day length in controlled-environment houses
• Warmer temperature – 26^oC is ideal
• Reduce number of feedings and particle size
• Temper amino acid nutrition (with caution). Low crude protein/high amino acid diets limit the increase in egg size.

Midnight feeding provides about 1-hour extra light per day and therefore stimulating feed consumption in the middle of the dark period. Having access to feed during this period improves eggshell quality via the supply of calcium during the time when shell calcification takes place. The extra light period is perceived by the bird to be part of the night. The dark period after the light period must be longer than the initial dark period, as the bird perceives the start of the day is the end of the longest period of darkness. Removing midnight feeding should be done gradually – 15 minutes per week, advised Dr Leeson.

Preventing calcium depletion

Also known as cage layer fatigue, calcium depletion is becoming more common in all strains due to high sustained egg output. Calcium deficiency in the feed leads to loss of medullary or long bone (a reservoir of about 4g of calcium) and increased bone fragility. It is commonly seen at 35-40 weeks of age, with a 1-2% occurrence. If the incidence is more than 2%, seek advice for your pre-lay nutrition.

The development of the medullary bones takes about 10 days and requires additional calcium. Pre-lay rations support a smooth transition from developer feed to layer feed, with 2-2.5% calcium, while the other nutrients are similar to a layer feed. Pre-lay rations help the birds to adapt to the high calcium content of layer feed and to maintain sufficient daily feed intake.

To prevent calcium depletion, Dr Leeson suggested:

• Optimise pre-lay calcium (Ca) and phosphorous (P) nutrition
• Intake of 1.5g Ca, 350-450mg available P/day for at least 7 days prior to first egg
• During early lay, ensure 3.5-4 g Ca and 420 mg available P/day
• Consider vitamin D₃ water treatment (150 IU/day, twice weekly)

Pre-lay diets provide the bird with the opportunity to deposit medullary bone. This bone deposition coincides with follicular maturation and is under the control of both estrogens and androgens. The latter hormone seems essential for medullary bone growth, and its presence is manifested in the growth and reddening of the comb and wattles. Consequently, there will be little medullary deposition, regardless of diet calcium level, if the birds are not showing comb and wattle development and this stage of maturity should be the cue for increasing the bird’s calcium intake.

Liver health

Excess energy relative to needs results in excess fat accumulation that is prone to oxidation. This is why you never see fatty liver haemorrhagic syndrome (FLHS) in poor-producing flocks. Layers normally have a very fatty liver, as 100% of egg yolk synthesis occurs in the liver.

The lower the fat content of the diet, the greater the stress/need to fat synthesis in the liver. With a low energy/low fat/carbohydrate diet FLHS is almost universal to varying degrees. One treatment is to add fat to the diet! Haemorrhage (not always FLHS) is inevitable with dietary omega-3s that are very prone to oxidation.

Dr Leeson recommended prevention/control for FLHS, which usually starts about weeks 36-40, including:

• +1.0 kg choline
• +0.5 kg methionine
• +100 IU vitamin E
• +30% does Hy-D because of impaired liver metabolism of vitamin D₃ (that can also impact calcium absorption)
• Add 2% dietary fat without change in diet energy level

 

***

EW Nutrition’s Poultry Academy took place in Jakarta and Manila in early September 2023. Dr. Steve Leeson, an expert in Poultry Nutrition & Production with nearly 50 years’ experience in the industry, was the distinguished keynote speaker.

Dr. Leeson had his Ph.D. in Poultry Nutrition in 1974 from the University of Nottingham. Over a span of 38 years, he was a Professor in the Department of Animal &Poultry Science at the University of Guelph, Canada. Since 2014, he has been Professor Emeritus at the same University. As an eminent author, he has more than 400 papers in refereed journals and 6 books on various aspects of Poultry Nutrition & Management. He also won the American Feed Manufacturer’s Association Nutrition Research Award (1981), the Canadian Society of Animal Science Fellowship Award (2001), and Novus Lifetime Achievement Award in Poultry Nutrition (2011).

 

Older laying birds are still a valuable asset, as long as they are managed for performance and productivity. Eggshell quality is one of the elements that, without proper management, can quickly deteriorate. It is therefore essential that the egg producer takes into account all the necessary elements for the formation of high-quality eggs.

 

The eggshell, in a nutshell

The eggshell represents ten percent of the entire egg, by weight[i]. For instance, a 60-gram egg contains approximately 6 g of shell. Out of this particular shell, approximately 95% is CaCO₃[ii], with a total of 2.3 g of Calcium (Ca).

But where does the calcium in the eggshell come from?

The Ca required for the eggshell is obtained, in variable proportions, directly from the feed or water additives (absorbed from the gut and transported via the blood to the shell gland), or from the bone (resorbed by osteoclasts and the Ca transported to the blood to the shell gland).

Maintaining eggshell quality and bone calcium: Mission Impossible?

Eggshell quality is often negatively correlated to bone strength[iii], most probably because body calcium is redirected to the shell to the detriment of the bones and the other way around. This impacts the long-term health of the skeleton; however, modern laying hens can maintain shell quality while preserving bone mineralization[iv].

60 to 75% of shell Ca is derived from the diet on shell-forming days

Approximately 60 to 75% of shell Ca is derived directly from the diet on shell-forming days[v]. This means that the greater the proportion of Ca coming directly from the feed or water additives, the better the eggshell quality can be. Therefore, the factors that can improve shell quality will also reduce the need to mobilize bone Ca and can also help to maintain skeletal health.

In old laying birds, generally after peak production, the ability to deposit Ca onto the shell remains relatively constant[vi], so an increase in egg size after peak production will tend to result in reduced shell quality. Dietary requirements for Ca tend to increase and those for phosphorus (P) tend to decrease as hens age.

Also, as hens age, the efficiency of Ca metabolism decreases[vii]. Increases in dietary Ca and a widening of the Ca:available P ratio are intended to counter this issue. Excess dietary P can also reduce shell quality[viii].

Because of its importance in Ca and P absorption from the gut, adequate dietary vitamin D activity must also be provided[ix]. Feeding of the vitamin D metabolite 25-OH vitamin D3 can help to maintain skeletal and shell quality in high-producing laying hens[x].

Ca metabolism is a complex game

Ca metabolism is regulated by various hormones such as calcitonin, 1,25-dihydroxyvitamin D3 (calcitriol), and parathyroid hormone. Estrogen, androgens, and prostaglandins also appear to have an important role in avian Ca metabolism.

 

Source: Ricardo (2008)

Egg formation and Ca requirements

 

Source

A hen ovulates approximately 15 to 75 minutes following oviposition[xi], and the ovum takes approximately 4.25 hours to reach the shell gland[xii], at which point calcification takes approximately 17 hours[xiii]. Hens generally lay eggs in the morning and early in the afternoon[xiv]. The hen can use the Ca and P made available through diet to recover medullary bone losses during the next 5 hours after oviposition.

Once the ovum reaches the shell gland, the demand for calcium naturally increases greatly as eggshell formation progresses. The highest eggshell mineral accretion takes place 5 – 15 hours after the egg enters the shell gland[xv], which normally happens later in the afternoon and during the night preceding egg laying.

Hourly Ca requirements for eggshell calcification

 

Ca dietary requirements vary with species, age, breeding status, and dietary levels of vitamin D. Egg-laying birds and growing birds require more Ca than adult non-breeding birds.

Common eggshell quality problems and causes

In many cases, the source of eggshell problems can be detected by recognizing the specific markers. For instance, cracked, soft-shelled or corrugated eggs can be caused by saline water, or the impact of mycotoxins; shell-les eggs can be caused by improper amounts of Ca, P, Mn or vitamin D3, as well as by infectious bronchitis or Newcastle disease IB. However, among the main causes of eggshell quality issues is heat stress.

In hot temperatures, increased respiration rates can cause an increase in CO₂ loss. The reduction of the pool of bicarbonate ions can result in respiratory alkalosis and an increase in blood pH[xvi]. A reduction in bicarbonate ions in the shell gland reduces the formation of CaCO₃ and decreases shell quality.

Under heat stress, birds will also tend to decrease their feed intake during the day to reduce diet-induced thermogenesis. Calcium intake is therefore also reduced, and shell quality decreases as a consequence.

3 solutions for eggshell quality in older layers

Midnight feeding in hot climates

At midnight, when temperatures are typically cooler, the addition of one to two hours of light can help the birds increase feed consumption[xvii]. Midnight feeding can also have the benefit of providing a dietary source of Ca to support eggshell formation during the night and reduce reliance on bone reserves[xviii].

Nutrition supplements

Along with Calcium, some micro-minerals can also influence eggshell quality. Zinc, Manganese and Copper act as cofactors of enzymes involved in the mineralization process during eggshell formation. Although European Union legislation restricts the use of high levels of these minerals, several studies in layers indicate increased egg shell resistance by increasing the dietary concentrations of microminerals. Using organic forms of Zinc, Manganese and Copper appears to be an alternative way to increase the absorption of these minerals, as organic forms appear to be more digestible than inorganic forms. Considering the high cost of organic minerals, a mix of organic and inorganic forms of critical minerals could be a better option.

Liquid Ca supplements

If a hen is fed a diet containing only a small-particle Ca sources, such as finely ground limestone, the intestine will be deprived of a source of Ca during the night, when demand for Ca is highest. At that point, the hen will be entirely reliant on bone Ca to support eggshell formation. A combination of Ca supplementation through water additives can be a good alternative as readily available Ca to the hen to support high Ca requirements during the late afternoon and through the night. Liquid Ca additives also offer further precise and user-friendly    application.

Stimuvital IP: a liquid solution from EW Nutrition

Stimuvital IP (formerly Shellimprover) is a liquid nutritional additive for laying hens, supporting the quality of eggshells and bone health. It contains a cocktail of Ca and vitamins whose benefits in laying birds are well proven through field studies, existing literature, and years of market experience.

Benefits proven in Australia field trial

22,500 layer birds were split into two equal-size groups, one of which (11250 birds) was supplemented with Stimuvital IP for 3 days every two weeks, starting from age 53^rd to 63^rd week. Improvements in eggshell thickness and strength could be noticed after the application of Shellimprover. Egg weight was consistent in Stimuvital IP -supplemented birds.3 days every fortnight by using the Easy@ system. In total, the 11250 birds received (2 (feed lines) x 3 (times per days) x 265ml x 3 (days) x 6 (week 53, 55, 57, 59, 61, 63) 28620mll of Stimuvital IP.

 

 

 

Benefits proven in China field trial

The field trial was carried out on a commercial layer farm. A control group and Stimuvital IP (Shellimprover) group had 50,000 birds each. Stimuvital IP was supplemented for 3 days every two weeks, starting from age 57^th to 62^nd week. The Stimuvital IP supplementation improved eggshell quality, including eggshell thickness, laying rate, and number of saleable eggs during the trial period.

 

 

 

Optimizing quantity, quality, and overall profitability for layer producers

Ca concentration in the blood is controlled by many interacting feedback loops that involve Ca, phosphate, PTH, vitamin D₃, and calcitonin. Supplementation of Vitamin D₃ can help maintain skeletal and shell quality in high-producing laying hens[xxiv].

Stimuvital IP offers an essential cocktail that caters to the additional requirements of Ca and vitamins in older laying birds. It thus supports Ca metabolism and eggshell quality. And, in the end, better eggshell quality reduces broken egg percentage and optimizes the number of salable eggs and profitability for layer producers.

 

Notes

[i] Pelicia et al., 2009; Bello and Korver, 2019

[ii] Nys et al., 2004

[iii] Orban and Roland Sr, 1990

[iv] Bello and Korver, 2019

[v] Driggers and Comar, 1949

[vi] Roland Sr et al., 1975

[vii] Wistedt et al., 2019

[viii] Miles et al., 1983

[ix] Wen et al., 2019

[x] Silva, 2017; Akbari Moghaddam Kakhki et al., 2019

[xi] Beuving and Vonder, 1981

[xii] Roberts, 2004

[xiii] Hincke et al., 2012

[xiv] Samiullah et al., 2016; Hunniford et al., 2017

[xv] Hincke et al., 2012

[xvi] Franco-Jimenez et al., 2007

[xvii] van Staaveren et al., 2018

[xviii] Harms et al., 1996

[xix] Chowdhury, 1990

[xx] Leach and Gross, 1983

[xxi] Zhang et al., 2017

[xxii] Atteh and Leeson, 1983

[xxiii] Atteh and Leeson, 1985

[xxiv] Silva, 2017; Akbari Moghaddam Kakhki et al., 2019

Full references are available upon request.