By Dr. Inge Heinzl, Editor EW Nutrition and
Marie Gallissot, Global Manager Feed Quality Solutions EW Nutrition

Antibiotic resistance is a growing global health concern, making infections more complicated to treat and increasing the risk of disease spread, severe illness, and death. While overuse and misuse of antibiotics are the primary causes, recent research has uncovered another unexpected contributor: mycotoxins. Among these, deoxynivalenol (DON), a toxin commonly found in contaminated grains, has been shown to significantly alter gut microbiota and promote antibiotic resistance. This article examines how DON impacts gut bacteria, influences antibiotic resistance, and highlights why this issue warrants urgent attention.

Mycotoxins – originators of antimicrobial resistance?

Actually, it would be logical…

Alexander Fleming discovered Penicillin when he returned after the summer holidays and saw that a mold had grown on the agar plate he had prepared. Around the mold, Staphylococcus was unable to proliferate. The reason was a substance produced by the mold – penicillin, which, like other toxins produced by molds, is a mycotoxin. In his article about the origin of antibiotics and mycotoxins, Shier (2011)

stated that antibiotics and mycotoxins share considerable similarities in structure, metabolic roles, and biosynthesis.

A short excursus to antimicrobial resistance

In general, the primary mechanisms of resistance involve the prevention or limitation of the antimicrobial substance’s uptake, modifying the drug target, inactivating the drug, or facilitating its discharge with efflux pumps.

There are two types of resistance: natural resistance, which is further divided into intrinsic and induced resistance, and acquired resistance.

Intrinsic resistance is a “characteristic” of a bacterial species and is not dependent on antibiotic exposure. An example is the reduced permeability of the outer membrane of gram-negative bacteria, which prevents certain antibiotics from entering.

Induced resistance, however, needs to be initiated by antibiotics. Here, multidrug-efflux pumps can be mentioned.

The third one, acquired resistance, refers to the process by which bacteria acquire genetic material, the resistance genes, from other bacteria that are resistant. The mechanisms include vertical transfer to daughter cells and horizontal transfer, such as the transfer from dead bacteria to living ones, by viruses, or the transfer of plasmids (Reygaert, 2018).

Deoxynivalenol (DON) promotes resistance in gut microbiota

A Chinese group of researchers (Deng et al., 2025) examined for the first time the influence of DON on the intestinal microbiota of chickens. One of the most alarming findings is DON’s ability to enhance antibiotic resistance. It contributes to this issue in several ways:

1. Encouraging resistant bacteria – By disrupting microbial balance, DON provides a survival advantage to bacteria that carry resistance genes.
2. Activating resistance genes – Studies suggest that DON can increase the expression of genes that help bacteria withstand antibiotics.
3. Enhancing gene transfer – Bacteria can share resistance genes through horizontal gene transfer. DON appears to promote this process, making antibiotic-resistant strains spread more rapidly.
4. Weakening antibiotic effectiveness – DON-induced changes in the gut environment can reduce the effectiveness of antibiotics, making treatments less successful.

A further indication that mycotoxins can enhance resistance is the significant overlap in the geographical distribution of antimicrobial-resistant bacteria and genes with that of mycotoxins, as noted by Deng et al.

Which protection mechanisms do bacteria have against mycotoxins?

In the case of mycotoxins, bacteria employ similar molecular mechanisms to those used against antibiotics. In an in vitro experiment, Hassan et al. (2019) challenged Devosia mutans, a gram-negative bacterium, with DON in the growth medium. DON inhibits protein synthesis, induces oxidative stress, and compromises cell membrane integrity in eucaryotic cells. Hassan et al. asserted three adaptive mechanisms as the response to the challenge:

1. Activation of cellular membrane proteins (adenosine 5’-triphosphate-binding cassette -ABC- transporters) responsible for the unidirectional transport of substrates, either outward or inward. These ABC transporters can work as drug efflux pumps.
2. Production of DON-specific deactivation enzymes, thereby engaging a toxin-specific pyrroloquinoline quinone-dependent detoxification pathway. This enables the bacterial isolate to transform DON to a non-toxic stereoisomer.
3. Upregulation of auxiliary coping proteins, such as porins (transmembrane proteins involved in metabolite exchange), glutathione S-transferases, and phosphotransferases, both of which are likely involved in the detoxification of xenobiotics.

Public health implications and preventive measures

Given the widespread presence of DON in food and animal feed, its potential role in antibiotic resistance poses a serious threat. The combination of increased bacterial resistance and weakened antibiotic efficacy could lead to more difficult-to-treat infections. This is particularly concerning in hospital settings, where antibiotic-resistant infections already cause high mortality rates.

To address the issue, several strategies can be implemented:

1. Reducing DON contamination: Implementing improved agricultural practices, such as crop rotation, the use of fungal-resistant crop varieties, and maintaining proper storage conditions, can help limit fungal growth and DON production.
2. Monitoring food and feed supply – Strict regulations and testing for DON contamination in grains and animal feed are essential to minimize human and animal exposure.
3. Effective mycotoxin risk management at feed mill and farm levels: Using tools such as MasterRisk and effective products combatting mycotoxins.
4. Maintaining gut health: A healthy diet rich in fiber, probiotics, and gut health-supporting feed supplements, such as Ventar D or products from the Activo line, may help counteract some of the adverse effects of DON on gut microbiota.
5. Developing new treatments: Research into alternative therapies and new antibiotics is crucial to combat the rise of antibiotic resistance.

Antimicrobial resistance: Be aware of the mycotoxins!

The connection between mycotoxins, such as DON, and antibiotic resistance underscores the need for a broader perspective on public health and food safety and once again brings the “One Health Concept” into focus. While antibiotic overuse remains the primary driver of resistance, environmental factors, such as exposure to mycotoxins, should not be overlooked. By increasing awareness, enhancing food safety regulations, and investing in research, we can take steps to mitigate this emerging threat and safeguard the effectiveness of antibiotics for future generations.

References:

Deng, Fengru, Chuying Yao, Linyu Ke, Meichan Chen, Mi Huang, Jikai Wen, Qingmei Chen, Jun Jiang, and Yiqun Deng. “Emerging Threat to Antibiotic Resistance: Impact of Mycotoxin Deoxynivalenol on Gut Microbiota and Clonal Expansion of Extensively Drug-Resistant Enterococci.” Environment International 197 (March 2025): 109353.
https://doi.org/10.1016/j.envint.2025.109353.

Hassan, Yousef I., Jian Wei He, Dion Lepp, and Ting Zhou. “Understanding the Bacterial Response to Mycotoxins: The Transcriptomic Analysis of Deoxynivalenol-Induced Changes in Devosia Mutans 17-2-E-8.” Frontiers in Pharmacology 10 (November 14, 2019).
https://doi.org/10.3389/fphar.2019.01098.

Reygaert, Wanda C. “An Overview of the Antimicrobial Resistance Mechanisms of Bacteria.” AIMS Microbiology 4, no. 3 (2018): 482–501.
https://doi.org/10.3934/microbiol.2018.3.482.

Shier, W. Thomas. “On the Origin of Antibiotics and Mycotoxins.” Toxin Reviews 30, no. 1 (January 28, 2011): 6–30.
https://doi.org/10.3109/15569543.2011.550862.

Smith, William P., Benjamin R. Wucher, Carey D. Nadell, and Kevin R. Foster. “Bacterial Defences: Mechanisms, Evolution and Antimicrobial Resistance.” Nature Reviews Microbiology 21, no. 8 (April 24, 2023): 519–34.
https://doi.org/10.1038/s41579-023-00877-3.

Dr. Inge Heinzl – Editor of EW Nutrition, and Dr. Merideth Parke – Global Application Manager for Swine, EW Nutrition

The first of the two articles focused on general aspects to be observed to achieve a particular stock of healthy and well-performing sows, as well as high productivity on the farm. In addition to general measures, feed supplements can be used to further support the sows. Phytomolecules with characteristics supporting gut and overall health are effective for this purpose.

Phytomolecules – how can they help?

Phytogenics, also known as phytomolecules, are plant-derived, natural bioactive compounds that promote livestock health and well-being, as well as improve growth performance and production efficiency. Phytomolecules encompass a diverse range of compounds, including terpenes, phenols, glycosides, saccharides, aldehydes, esters, and alcohols.

The literature describes some of their effects, including stimulation of digestive secretions, immune stimulation and anti-inflammatory activity, intestinal microflora modulation, and antioxidant effects (Durmic and Blanche, 2012; Ehrlinger, 2007; Zhao et al., 2023), as well as estrogenic and hyperprolactinemic properties (Farmer, 2018) and effects on colostrum and milk porcine sensory profiles (Val-Laillet et al., 2018). They represent exciting antibiotic alternatives in swine production (Omonijo et al., 2018).

1. Phytomolecules modulate intestinal microbiota

Phytomolecules are microbiome modulators through different mechanisms. They can directly impact pathogenic bacteria by damaging the cell membrane, cell wall, or cytoplasm, interrupting the anion exchange, resulting in changes to cellular pH, and inhibiting the cell’s energy production system. Additionally, phytomolecules interfere with the virulence capacity of pathogenic bacteria through the indirect quorum quenching mechanism. (Rutherford and Bassler, 2012).

The favorable consequence of this differential microbial modulation is maintaining gut microbiome diversity, shifting it to a bacterial population with reduced pathogenic and increased beneficial microbes.

Proof of Ventar D’s pathogen-inhibiting effect

An in vitro study evaluated the effect of Ventar D on pathogenic Clostridium perfringens and beneficial Lactobacillus spp.

Process

To test the effect of Ventar D on four different beneficial Lactobacillus spp., and pathogenic Clostridium perfringens, the phytogenic formulation (Ventar D) was added to the respective culture medium in the following concentrations: 0 µg/mL – control, 500 µg/mL (only C. perfr.), 750 µg/mL, 1000 mg/mL (only C. perfr.), and 1250 µg/mL.

After cultivating the bacteria in the culture medium, the colony-forming units (CFU) were counted.

Results and discussion

The study demonstrated a dose-dependent decrease in the Clostridium perfringens population. At the lowest tested concentration (500 µg/mL), Ventar D’s antimicrobial effect was already detectable; at 750 µg/mL, scarce colonies were observed; and at 1000 µg/mL, C. perfringens could no longer grow.

In contrast, even at higher concentrations of Ventar D, the beneficial L. agilis S73 and L. agilis S1 populations were only mildly affected, and L. casei and L. plantarum were unaffected.

These findings confirm the differential antimicrobial activity of Ventar D’s formulation, specifically a bactericidal effect on pathogenic C. perfringens populations and a mild to no inhibition of beneficial Lactobacillus spp.

2. Phytomolecules improve intestinal integrity

The gut barrier is semipermeable and is responsible for immune sensing and regulating the movement of nutrients and undesirable microbes and substances.

The “gatekeepers” are tight junctions (TJ), adherent junctions (AJ), and desmosomes situated between the intestinal enteric cells (IEC). The tight junctions regulate the transport of small molecules and ions. The adherent junctions and desmosomes maintain the integrity of the intestinal barrier by keeping the IECs together through cell-adhesion bonds.

Oxidative stress resulting from factors such as heat stress or fat oxidation in the feed, as well as dysbacteriosis caused by changes in diet, out-of-feed events, poor dietary formulation, or bacterial contamination, can compromise the integrity of these critical adhesions and junctions between enterocytes.

The support of these tight junctions prevents bacteria and toxins from passing into the organism. Besides reducing disease occurrence, it also reduces the activation of the immune system and inflammatory processes. Ingested nutrients can be used for growth and need not be spent for the defense of the organism.

Proof of Ventar D’s gut barrier-stabilizing effect

An experiment was conducted to determine the level of tight junction gene expression biomarkers closely related to gut integrity.

Process

The experiment was conducted in broilers. They were fed 100 g of Ventar D/ t of feed, and the gene expression of Claudin and Occludin was measured (the higher the gene expression, the higher the level of gut barrier damage).

Results

The lower levels of both gut tight junction gene expression biomarkers, Claudin and Occludin, in Ventar D-supplemented birds support a lower level of damage and a more robust gut barrier function (Figure 3).

3. Phytomolecules act as antioxidants

As mentioned, oxidative stress can disrupt gut barrier function and negatively impact the health of sows and piglets. Therefore, it is vital to scavenge reactive oxygen species (ROS) to reduce the damage these free radicals can cause to enterocytes and tight junctions.

Proof of Ventar D’s antioxidant effect in vitro

In this case, an in vitro trial was conducted to show Ventar D’s antioxidant effects.

Process

Ventar D’s antioxidant activity was tested in vitro using the ORAC (Oxygen Radical Absorbent Capacity) test. The ORAC test measures the antioxidant activity of a compound compared to that of the Vitamin E analog Trolox.

Result

The components in Ventar D demonstrated its capacity as an antioxidant, with a more substantial effect than the Vitamin E analog Trolox (see Figure 4).

4. Phytomolecules decrease inflammation

In intensive production, animals face daily inflammation associated with various stressors, including gut incidents and intestinal dysbiosis, social hierarchy-associated fighting resulting in musculoskeletal or skin injuries, farrowing and lactation trauma to reproductive organs, and diseases affecting any system in the pig.

Animals with high-performance expectations, such as gestating, farrowing, and lactating sows, are particularly susceptible to high nutrient diversion, which can lead to inflammation and activation of the immune system. To mitigate the excessive continuation of inflammatory processes, phytomolecules with anti-inflammatory effects can be utilized.

Proof of Ventar D’s anti-inflammatory effect in vitro

The anti-inflammatory effect of Ventar D was shown in an in vitro trial conducted in the Netherlands.

Process

For the trial, cells from mice (Murine macrophages, RAW264.7) were stressed with lipopolysaccharides (LPS, Endotoxin) from E. coli O111:B4 (0.25 µg/ml) to provoke an immune response. To evaluate the effects of Ventar D, two different concentrations (50 and 200 ppm) were tested, and the levels of NF-κB, IL-6, and IL-10 were determined. IL-6 and IL-10 could be measured directly using specific ELISA kits, whereas, in the case of NF-κB activity, an enzyme induced by NF-κB (secreted embryonic alkaline phosphatase – SEAP) was used for measurement. The trial design was as follows (Figure 5):

Results

The trial results showed a dose-dependent reduction of NF-κB activity in LPS-stimulated mouse cells, with 11% and 54% reductions at 50 and 200 ppm Ventar D, respectively. The pro-inflammatory cytokine IL-6 was downregulated, and the anti-inflammatory cytokine IL-10 was upregulated by 84% and 20%, respectively, resulting in a decrease in the IL-6 to IL-10 ratio. This ratio is essential in balancing the pro- and anti-inflammatory outcomes of cellular signaling.

5. Phytomolecules improve production performance and efficiency

The intensive production systems of today encompass many factors that create stress in the animals. Phytomolecules exhibiting the positive characteristics mentioned in points 1 to 4 result in better performance in animals.

In pigs in suboptimal conditions, the antimicrobial effect of phytomolecules is the most important. However, in pigs held under optimal conditions and with extraordinary growth, the antioxidant and anti-inflammatory effects are most relevant. Anabolic processes, driven by strong growth, increase oxidative stress, while non-infectious inflammations burden the immune system.

Proof of Ventar D’s performance-promoting effect in pigs

To evaluate growth-promoting effects in pigs, a study was conducted on a commercial farm in the United States.

Process

A total of 532 approx. 24-day-old weaned piglets were housed in 28 pens, each containing 19 non-castrated males or gilts. Piglets were blocked by body weight and fed a three-phase feeding program (Table 1). Phase 1 and 2 diets were pellets, and phase 3 was mash. Diets were based on corn and soybeans, and a concentrate including soy protein concentrate, whey permeate, and fish meal was added in phases 1 and 2, at a ratio of 50% of the total feed in phase 1 and 25% in phase 2. No feed medication was used in this trial.

Table 1: Feeding scheme and product application

Trial groups	Feeding Phase 1 (day1 – day 14)	Feeding Phase 2 (day 15 – day 24)	Feeding Phase 3
Control	No additive	No additive	No additive
Ventar D	Ventar D 200 g/MT	Ventar D 200 g/MT	Ventar D 200 g/MT

Results

Adding Ventar D increased final body weight and improved FCR (see Figures 8 to 10). Furthermore, the addition of Ventar D to the feed reduced mortality.

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Figures 8-10: Performance of piglets fed Ventar D in comparison to a negative control

Phytomolecules can help to keep sows healthy and productive

Intensive animal production places a significant strain on animal organisms. High stocking density often accompanies high pathogenic pressure and stress, and high growth performance can lead to increased oxidative stress and inflammation. It isn’t easy to keep all challenges under control. However, phytomolecules can be a solution as their modes of action cover different relevant topics.

References

Durmic, Z., and D. Blache. “Bioactive Plants and Plant Products: Effects on Animal Function, Health and Welfare.” Animal Feed Science and Technology 176, no. 1–4 (September 2012): 150–62. https://doi.org/10.1016/j.anifeedsci.2012.07.018.

Ehrlinger, Miriam. “Phytogene Zusatzstoffe in der Tierernährung.” 2007. https://edoc.ub.uni-muenchen.de/6824/1/Ehrlinger_Miriam.pdf

Farmer, Chantal. “Nutritional Impact on Mammary Development in Pigs: A Review.” Journal of Animal Science 96, no. 9 (June 15, 2018): 3748–56. https://doi.org/10.1093/jas/sky243.

Omonijo, Faith A., Liju Ni, Joshua Gong, Qi Wang, Ludovic Lahaye, and Chengbo Yang. “Essential Oils as Alternatives to Antibiotics in Swine Production.” Animal Nutrition 4, no. 2 (June 2018): 126–36. https://doi.org/10.1016/j.aninu.2017.09.001.

Rutherford, S. T., and B. L. Bassler. “Bacterial Quorum Sensing: Its Role in Virulence and Possibilities for Its Control.” Cold Spring Harbor Perspectives in Medicine 2, no. 11 (November 1, 2012). https://doi.org/10.1101/cshperspect.a012427.

Val-Laillet, David, J Stephen Elmore, David Baines, Peter Naylor, and Robert Naylor. “Long-Term Exposure to Sensory Feed Additives during the Gestational and Postnatal Periods Affects Sows’ Colostrum and Milk Sensory Profiles, Piglets’ Growth, and Feed Intake1.” Journal of Animal Science, June 29, 2018. https://doi.org/10.1093/jas/sky171.

Zhao, Bi-Chen, Tian-Hao Wang, Jian Chen, Bai-Hao Qiu, Ya-Ru Xu, Qing Zhang, Jian-Jie Li, Chun-Jiang Wang, Qiu-Feng Nie, and Jin-Long Li. “Effects of Dietary Supplementation with a Carvacrol–Cinnamaldehyde–Thymol Blend on Growth Performance and Intestinal Health of Nursery Pigs.” Porcine Health Management 9, no. 24 (May 23, 2023). https://doi.org/10.1186/s40813-023-00317-x.

VISBEK, 20 March 2025 – EW Nutrition, a global provider of animal nutrition solutions, announced today that it has acquired the majority stake in Austrian company Green Innovation, producer and patent-holder of several in-feed solutions in the gut health space.

Green Innovation, based in Innsbruck, and EW Nutrition, headquartered in Northern Germany, have signed the agreement on Thursday 20 March. “By acquiring 52% of our company, EW Nutrition implicitly recognizes the strength of our solutions, know-how, and technologies. We are happy that we found such a solid and dynamic partner, and we are excited to work together for the next level of animal gut health,” says Alexander Herbst, CEO of Green Innovation.

For EW Nutrition, solutions such as Ventar D, Pretect D, Activo or Activo Liquid have already carved an important space in the gut health arena. “With the addition of Green Innovation’s solutions, we see a great opportunity to complete our gut health portfolio and to position ourselves for the future. Challenges are evolving and so our solutions must evolve as well,” notes CEO Jan Vanbrabant.

With the fourth acquisition in the last five years, the company shows its continued drive toward further development and expansion. The new deal gives EW Nutrition majority ownership of innovative brands such as Argat or Oxilem, as well as patents, know-how, and a number of team members who will manage the transfer together with their legacy counterparts.

Green Innovation customers will be supported to the usual high standards, while the assets, brands, and go-to-market will be transitioned to EW Nutrition in the coming period.

The financial details of the sale remain confidential.

***

About EW Nutrition

EW Nutrition is a global animal nutrition company that offers comprehensive, customer-focused solutions for gut health management, feed quality, digestibility, and more.

Contact: info@ew-nutrition.com

Dr. Inge Heinzl – Editor of EW Nutrition and
Dr. Merideth Parke – Global Application Manager for Swine, EW Nutrition

Sow mortality critically impacts herd performance and efficiency in modern pig production. Keeping the sows healthy is, therefore, the best strategy to keep them alive and productive and the farm’s profitability high.

Rising mortality rates are alarming

In recent years, sow mortality has increased across pig-raising regions in many countries. Eckberg’s (2022) findings from the MetaFarms Ag Platform (including farms across the United States, Canada, Australia, and the Philippines) determined an increase of 66.2% between 2012 and 2021.

What can be done to decrease mortality rates?

Several measures can be taken to reach a particular stock of healthy and high-performing sows. In the following, the main remedial actions will be explained.

1. Determination of the cause of death

If a sow is dead, it must first be clarified why it has died. If the sow is culled, the reason for this decision is usually apparent. If the sow suddenly dies, investigations, including a thorough postmortem, are extremely valuable to determine the cause of death. Kikuti et al. (2022) provided a collection of the most-occurring causes of death in the years 2009 to 2018. As often, no necropsy is conducted, and the causes of death remain unclear, as shown by the high numbers of “other”. Locomotory (e.g., lameness) and reproductive (e.g., prolapse, endotoxemic shock from retained fetuses) incidents account for approximately half of the recorded sow mortalities (Kikuti et al., 2022), especially during the first three parities. (Marco, 2024).

Evaluating detailed breeding history together with the cause of death will provide perspective and assist veterinary, nutritionist, and husbandry teams with interventions to prevent similar events and early sow mortality.

Selection of the gilts

After selecting the best genetics and rearing the gilts under the best conditions, further selection must focus on physical traits such as structure, weight, height, leg, and hoof integrity.

Additionally, as we have more and more group housing for sows, the selection for stress resilience can positively impact piglet performance (Luttmann and Ernst, 2024). The following table compares stress-resilient and stress-vulnerable sows concerning piglet performance and shows the piglets of the vulnerable sows with worse performance.

Table 1: Influence of stress resilience on performance (Luttmann and Ernst, 2024)

Trait	SR	SV	p-Value
Birth weight (kg)	1.350 ± 0.039	1.246 ± 0.041	0.083
Wean weight(kg)	6.299 ± 0.185	5.639 ± 0.202	0.033*
Suckling ADG (kg/d)	0.191 ± 0.005	0.165 ± 0.005	0.004**

Least square means and standard error of stress resilient (SR) and stress vulnerable (SV) for each trait; significance threshold of p<0.05 with * indicating 0.01<p<0.05, ** indicating 0.001<p<0.01

How to manage the gilts best

The management of the gilts must consider the following:

1. Age at first estrus should be <195 days:
   Gilts having their first estrus earlier show higher daily gain and usually higher lifetime productivity. In a study conducted by Roongsitthichai et al. (2013), sows culled at parity 0 or 1 exhibited first estrus at 204.4±0.7 days of age, while those culled at parity ≥5 exhibited first estrus at 198.9±2.1 days of age (P=0.015).
2. Age at first breeding should lay between 200 and 225 days:
   If the sows are bred at a higher age, they have the risk of being overweight, leading to smaller second-parity litters, longer wean-to-service intervals, and shorter production life.
3. The body weight at first mating should be between 135 and 160 kg:
   To reach this target within 200-225 days, the gilts must have 600-800 g of average daily gain. Breeding underweight gilts reduces first-litter size and lactation performance. Overweight gilts (>160 kg) face higher maintenance costs and locomotion issues.
4. The number of estruses at first mating should be 2 or 3:
   Accurately track estrus and breed on the second estrus. Research shows that delaying breeding to the second estrus positively affects litter size. Only delay breeding to the third estrus to meet minimum weight targets.

Housing

Gestating sows are more and more held in groups. Understanding the process of group housing is essential for success. The following graphic shows factors impacting successful grouping.

If the groups are not well-established yet, the stress levels among sows are higher, leading to

• More leg injuries due to aggressive behavior or fighting for resources
• Higher rates of abortions and returns to service
• Reduced sow performance, including decreased productivity, lower milk yield, and poor piglet growth due to compromised immune function and overall health

To mitigate stress in group housing, it is crucial to implement proper group management practices, which include gradual introductions, maintaining stable social structures, and ensuring adequate space and resources. This helps promote a calmer environment, improving animal welfare and herd performance.

Responsible on-farm pig care

Caregivers must be well-trained and equipped to provide high-quality care. Insufficient or unskilled pig caregivers can significantly affect the growth and development of prospective replacement gilts, ultimately influencing their suitability for the breeding herd:

• Growth Rates: Suboptimal nutrition and health management result in slower growth rates and poor body condition.
• Health Issues: Unskilled handling may increase the risk of disease transmission, injuries, and stress, all of which can adversely affect growth and overall development.
• Behavioral Problems: Poorly managed environments can increase aggression and competition among animals, hindering growth and health.
• Selection Criteria: Ineffective growth and health monitoring can result in misjudging the potential of gilts, leading to the selection of less suitable candidates for the breeding herd.

Table 2: Influence of handling on growth performance and corticosteroid concentration of female grower pigs from 7-13 weeks of age (Hemsworth et al., 1987)

	Unpleasant	Pleasant	Inconsistent	Minimal
ADG (g)	404a	455b	420ab	4.58b
FCR (F:G)	2.62b	2.46a	2.56b	2.42a
Corticosteroid conc (ng/mL)	2.5a	1.6b	2.6a	1.7b

Responsible on-farm pig care is crucial to keep sows healthy and performing. Poor sow observations (e.g., failure to identify stressed, anorexic, or heat-stressed sows) or inappropriate farrowing interventions can directly influence sow health and potentially reduce subsequent performance or mortality. On the contrary, rapid and proactive identification of sows needing intervention can save many animals that would otherwise die or need to be culled.

Keeping sows healthy and performing is manageable

The maintenance of sows’ health is a challenge but manageable. Observing all the points mentioned, from selecting the right genetics over rearing the piglets under the best conditions to managing the young gilts, can help prevent disease and performance drops. For all these tasks, farmers and farm workers who do their jobs responsibly and passionately are needed. The following article will show nutritional interventions supporting the sow’s gut and overall health.

References:

Eckberg, Bradley. “2021 Sow Mortality Analysis.” National Hog Farmer, February 3, 2022. https://www.nationalhogfarmer.com/hog-health/2021-sow-mortality-analysis.

Hemsworth, P.H., J.L. Barnett, and C. Hansen. “The Influence of Inconsistent Handling by Humans on the Behaviour, Growth and Corticosteroids of Young Pigs.” Applied Animal Behaviour Science 17, no. 3–4 (June 1987): 245–52. https://doi.org/10.1016/0168-1591(87)90149-3.

Kikuti, Mariana, Guilherme Milanez Preis, John Deen, Juan Carlos Pinilla, and Cesar A. Corzo. “Sow Mortality in a Pig Production System in the Midwestern USA: Reasons for Removal and Factors Associated with Increased Mortality.” Veterinary Record 192, no. 7 (December 22, 2022). https://doi.org/10.1002/vetr.2539.

Marco, E. “Sow Mortality: How and Who? (1/2).” Pig333.com Professional Pig Community, March 18, 2024. https://www.pig333.com/articles/sow-mortality-how-are-sows-dying-which-sows-are-dying_20105/.

Luttmann, A. M., and C. W. Ernst. “Classifying Maternal Resilience for Improved Sow Welfare, Offspring Performance.” National Hog Farmer, September 2024. https://informamarkets.turtl.co/story/national-hog-farmer-septemberoctober-2024/page/5.

Roongsitthichai, A., P. Cheuchuchart, S. Chatwijitkul, O. Chantarothai, and P. Tummaruk. “Influence of Age at First Estrus, Body Weight, and Average Daily Gain of Replacement Gilts on Their Subsequent Reproductive Performance as Sows.” Livestock Science 151, no. 2–3 (February 2013): 238–45. https://doi.org/10.1016/j.livsci.2012.11.004.

Conference Report

Nowadays, climate change is an omnipresent topic. Extreme weather events, such as high temperatures and heavy rainfall, are becoming more frequent, and there has been a rapid increase in greenhouse gas concentrations since the 1850s. Climate change will also have consequences for the pig industry. Dr. Jan Fledderus, Schothorst Feed Research, discussed upcoming issues for the pig industry at EW Nutrition’s Swine Academy.

Shift in mycotoxin-producing fungi

Climate change is likely to expand the geographical range of mycotoxin-producing fungi, exposing new crops and areas previously considered low risk to higher contamination levels. For instance, regions in South and Eastern Europe have reported increased occurrences of aflatoxins due to hotter and drier conditions favoring Aspergillus flavus over Fusarium species.

European Green Deal

The European Commission has adopted the European Green Deal, a comprehensive policy initiative to address climate change and promote sustainability within the European Union (EU). It sets ambitious targets and outlines a roadmap for reducing greenhouse gases by at least 55% by 2030, compared to 1990 levels, and achieving climate neutrality by 2050. The EU’s primary goal is to ensure food security while reducing environmental and climate footprint.

The EU regulation on deforestation-free products includes soybeans and palm oil. The objective is to guarantee that the products EU citizens consume do not contribute to deforestation or forest degradation worldwide. Effective 1 January 2026, all imported soy must be free of deforestation. This means soybeans must be from areas not deforested since 1 January 2021.

The Green Deal will affect pig production

While it is still early to fully assess the impacts of the European Green Deal on pig farmers, it is clear that regulatory changes, economic pressures, and shifts in consumer behavior will shape the future of pig farming in the EU. Several potential consequences are still being assessed, including:

• Halving nutrient losses, particularly nitrogen, influences the eutrophication of natural areas and surface water, which will likely require pig farmers to adjust their feeding strategies and potentially reduce herd sizes.
• The use of food waste and by-products, such as wheat bran, in pig diets will be encouraged, promoting a circular economy approach that minimizes waste and enhances resource efficiency.
• Costs (notably related to feed) are likely to increase due to manure management and a reduction in crop production due to stricter environmental regulations.
• Farmers may need to invest in more sustainable practices and technologies to comply with new regulations, which could strain finances unless supported by subsidies or compensatory payments.
• Reduced supply and higher consumer prices for pigmeat products.
• Encouraging a shift towards plant-based diets in humans, which may reduce demand for pork (and other animal proteins).
• There may be opportunities for the pig industry to develop premium products that meet sustainability criteria or cater to specific consumer preferences.

Defining sustainability

It is necessary to apply a uniform method to calculate sustainability parameters and define objectives for “sustainable pig feed.” The Global Feed LCA Institute (GFLI) is the global standard for raw material parameters. It gives data by different methods to calculate carbon dioxide (feed/food), with detailed data per country of origin, including peat oxidation. It includes 16 environmental impact categories.

Climate-neutral pig production

How does this impact pig production? Firstly, feed contributes 50-70% of CO₂ equivalents/kg of pigmeat. Secondly, it is essential to have a uniform method to calculate the CO₂ equivalents/kg of pigmeat. Currently, there are no financial benefits for pig farmers to improve sustainability.

Based on scenario calculations, Dr. Fledderus concluded that it is challenging to realize ‘zero emissions’ and that improving on all environmental impact parameters is not realistic. Formulating pig diets to reduce CO2 equivalents to produce ‘green pork’ increases feed costs. The obvious question is, who will pay for this?

 

EW Nutrition’s Swine Academy took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Jan Fledderus, Product Manager and Consultant at the S&C team at Schothorst Feed Research, one of the founders of the Advanced Feed Package and with a strong focus on continuously improving the price/quality ratio of the diets for a competitive pig sector, was a reputable guest speaker in these events.

Conference Report

Unlike humans and most mammals, piglets do not receive any maternal immunoglobulins (antibodies) via the placenta. Therefore, it is vital for piglets to receive maternal antibodies via the colostrum within 24 hours of birth. Otherwise, they are more vulnerable to illnesses in their early stages of life. In situations where piglets do not receive enough colostrum, such as due to large litter sizes or weak sows following a prolonged farrowing — supplemental colostrum or IgY products can provide essential immune protection.

In the following, Dr. Shofiqur Rahman describes the innovative role of IgY – yolk immunoglobulins in enhancing swine health.

IgY – modes of action

IgY is an antibody found in egg yolk. It is an entirely natural product; each egg contains approximately 100 mg of IgY. These egg-derived antibodies primarily function in the gut through several mechanisms:

• Adherence inhibition – IgY antibodies bind to specific structures on the surface of pathogens (such as fimbriae, flagella, and lipopolysaccharides), preventing them from adhering to the intestinal mucosa and blocking the initial stages of infection. This is particularly significant for enterotoxigenic E. coli (ETEC), which causes piglet diarrhea by attaching to intestinal cells.
• Neutralization – IgY can neutralize toxins produced by pathogens, preventing them from exerting harmful effects on host cells.
• Agglutination – IgY promotes the clumping of pathogens by binding them together, effectively immobilizing them, and facilitating their removal from the animal’s gut.
• Cell damage – IgY can damage the integrity of bacterial cell walls leading to cell lysis and reduced bacterial viability.

Furthermore, because these pathogens are bound in complexes with IgY and eliminated through feces in an inactivated form, IgY helps prevent environmental re-infection through manure.

IgY and IgG – functional differences

Both IgY and Immunoglobulin G (IgG) (IgG, the most abundant immunoglobulin in mammals) are antibodies. They, however, exhibit significant differences due to their distinct structural characteristics. “IgY, for instance, does not activate the complement system, a key function of IgG that enhances immune responses against infections. Additionally, IgY promotes more rapid phagocytosis and reduces inflammation compared to IgG. These effects contribute to energy conservation, thereby facilitating improved animal growth performance,” he explained.

IgY is more hydrophobic than IgG, which increases its stability and resistance to proteolytic degradation. This property is beneficial for maintaining its functionality in the gastrointestinal tract.

Production and quality control

IgY develops in hens in response to the pathogens they encounter, regardless of their relevance to the hens themselves. For instance, hens immunized with an infectious pathogen affecting pigs can produce IgY, effectively preventing the disease caused by that pathogen.

There are different methods of IgY production. One possibility is to hyperimmunize the hens simultaneously with multiple antigens. This method seems convenient, but it does not produce products with standardized levels of immunoglobulins for each antigen.

Another approach involves immunizing different groups of hens, each with a single antigen (e.g., transmissible gastroenteritis virus, rotavirus, E. coli) that commonly challenges piglets during the first weeks of life. The immunoglobulin content is then quantified, and the resulting egg powders are spray-dried, pasteurized, and mixed. This process yields an IgY product with standardized amounts of specific immunoglobulins that exhibit high affinity for the target pathogens.

One health application in swine

“The benefits of IgY have been demonstrated through extensive trials and commercial experiences, highlighting its potential for various applications not only in swine but also in other animals and humans,” said Dr. Rahman.

Due to concerns about antibiotic resistance, regulatory and consumer scrutiny increased over the use of in-feed antibiotics. IgY can serve as an effective and natural alternative for improving overall gut health, reducing the incidence and severity of diarrhea, reducing morbidity during the critical pre- and post-weaning periods, and, thereby, increasing performance.

Unlike antibiotics, which can indiscriminately kill both harmful and beneficial bacteria, IgY selectively targets specific pathogens. This selective action helps maintain a balanced gut microbiome, which is crucial for overall health and digestion in piglets. Disruption of the gut microbiota by antibiotics can lead to issues such as antibiotic-associated diarrhea and increased susceptibility to opportunistic infections due to the loss of beneficial microbes.

In contrast to antibiotics, IgY targets multiple antigenic sites on pathogens, requiring various genes for their protection, thereby avoiding resistance issues among pathogenic microorganisms. Additionally, IgY is effective not only against bacteria but also demonstrates significant efficacy against viruses and coccidia.

Conclusion

Dr. Rahman concluded that “the use of IgY as a passive immunization strategy, incorporated into a holistic approach to reducing piglet diarrhea, offers a safe and natural alternative to traditional antibiotics, particularly in the light of rising antibiotic resistance and the need for effective treatments also for viral diseases.”

EW Nutrition’s Swine Academy took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Shofiqur Rahman, Senior Researcher at the Immunology Research Institute Gifu (IRIG) in Japan was one of the highly experienced speakers of EW Nutrition. Originally a microbiologist, Dr. Rahman focuses on researching and developing IgY products for Human, Animal, Pet, Fish, Plant, and Environmental health.

By Ajay Bhoyar, Senior Global Technical Manager, EW Nutrition

Gut health is pivotal to profitable poultry production, as the gastrointestinal tract (GIT) enables nutrient digestion and absorption while acting as a defense against pathogens. A healthy gut improves feed conversion, boosts immune resilience, and reduces reliance on antimicrobials—critical in the fight against antimicrobial resistance (AMR). With AMR posing significant threats to public health and animal agriculture, strategies like biosecurity, sustainable management, and effective dietary interventions are gaining traction. Feed enzymes have emerged as essential tools for managing feed costs, mitigating anti-nutritional factors, and improving nutrient utilization. Among these, feed enzymes like xylanase stand out. By breaking down xylan, a major component of non-starch polysaccharides (NSPs) in plant-based feed ingredients, xylanase reduces gut viscosity, enhances nutrient utilization, and supports optimal gut health and productivity. This article explores the innovative application of novel GH10 xylanases, such as Axxess XY, as a sustainable solution for improving feed efficiency and gut health in poultry production.

Xylanase in Poultry Nutrition

Xylanase plays a pivotal role in enhancing nutrient availability by addressing the limitations of endogenous enzyme synthesis in poultry. Xylanase enzymes belong to the carbohydrase class, catalyzing the breakdown of xylan, a major NSP in plant-based feed ingredients. They hydrolyze xylan into simple sugars like arabino-xylo-oligosaccharides (AXOs) and xylo-oligosaccharides (XOs), reducing the encapsulation of nutrients and digesta viscosity. These actions improve overall nutrient digestibility and bird performance.

Fig.1: Arabinoxylans – anti-nutrient mode of action in chicken

The primary benefit of feed xylanase lies in its ability to reduce digesta viscosity. By partially hydrolyzing NSPs in the upper digestive tract, xylanase ensures better nutrient absorption in the small intestine. Studies (Matthiesen et al., 2021; Choct & Annison, 1992) confirm that reduced viscosity enhances feed digestibility, leading to improved performance in poultry. Further, to realize the optimum benefits, it is crucial that xylanase efficiently degrades both soluble and insoluble arabinoxylans. The insoluble arabinoxylans are part of the cell wall structure of plant cells, resulting in a cage effect, entrapping nutrients like starch and protein. Effectively breaking down insoluble arabinoxylans ensures that the nutrients trapped in plant cell walls are released for growth and production.

Mechanisms Supporting Gut Health

Viscosity Reduction

High NSP content increases digesta viscosity and slows digestion and nutrient absorption. Soluble arabinoxylan is not digested in the small intestine of broilers. It produces a viscous chime, leading to the proliferation of pathogenic bacteria, intestinal inflammation, impairment of barrier function in the intestine, and severe intestinal lesions (Teirlynck et al., 2009). Xylanase mitigates this by breaking down xylans, a major component of NSPs in common feed ingredients. This results in a better flow of digesta and reduced energy losses.

Microbial Metabolites

Xylo-oligosaccharides (XOS) can also be produced in the intestine of monogastric animals to some extent when exogenous enzymes, such as xylanase, are added to the feed (Baker et al., 2021).

The XOS generated by xylanase action on arabinoxylans can act as prebiotics, fostering beneficial bacteria like Lactobacillus and Bifidobacterium, which can outcompete harmful species. XOS can positively impact the gut microbiota, enhance short-chain fatty acid (SCFA) production, stimulate immune activity in the gastrointestinal tract, and improve energy utilization.

Fig. 2. Axxess XY improved beneficial microbes and reduced the clostridial population in broilers.

Barrier Function

By lowering inflammation and irritation in the intestine, xylanase helps maintain gut integrity, reducing the risk of pathogen translocation from the intestinal lumen. In a broiler study, xylanase decreases epithelial apoptosis index, up-regulates tight junction gene expression, and inhibits mucin synthesis in the small intestine, likewise alleviating the intestinal mucosal barrier impairment from Clostridium perfringens challenge (Liu et al., 2012).

Practical Considerations for Xylanase Use

Enzyme Stability

Enzymes are proteins that tend to lose their catalytic activity at high temperatures. When exposed to excessive heat, an enzyme’s protein structure can irreversibly unfold, disrupting its active site and causing loss of function. Therefore, ensuring enzyme stability during feed processing is critical for maintaining its activity in the intestine. Intrinsically heat-stable enzymes have an inherent ability to withstand higher temperatures without the need for a protective coating and are immediately available for action upon ingestion.

Feed Composition

Xylanase efficacy is influenced by diet composition, particularly the NSP content and the presence of xylanase inhibitors in common feedstuffs. It is important to choose a xylanase that can resist the activity of xylanase inhibitors and is effective against both soluble and insoluble arabinoxylans.

The recommended energy matrix value for the xylanase enzyme should be used while formulating the feeds to create energy-deficient diets to reap the full benefits of xylanase use.

Optimal Dosage

Proper dosing is essential to maximizing the benefits of feed enzymes while avoiding unnecessary costs. It is important to follow manufacturers’ recommendations and avoid underdosing an enzyme.

GH10 Xylanases: The Superior Choice for Animal Nutrition

Most feed xylanases are classified into glycoside hydrolase families 10 (GH10) and 11 (GH11) based on their substrate specificity, catalytic action, and structural features.

Why GH10 Xylanases Are More Effective

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1. Broader Substrate Specificity:

   Unlike GH11 xylanases, GH10 xylanases can effectively hydrolyze both soluble and insoluble xylan substrates. This broader activity ensures an efficient breakdown of xylans in a wide range of feed ingredients.

2. Higher Catalytic Efficiency:

   GH10 enzymes cleave xylan at substituted regions, yielding shorter xylo-oligosaccharides that can positively impact gut health and maximize nutrient availability.

3. Thermostability:

   Feed processing often involves high temperatures during pelleting. Axxess XY, a GH10 family xylanase, demonstrates remarkable thermostability, maintaining over 85% activity even at 95°C for extended conditioning times. This resilience ensures consistent enzyme performance during feed manufacturing and digestion.

Fig.3: Optimum recovery of Axxess XY at elevated conditioning time and temperatures

Novel Applications of Axxess XY: A GH10 Xylanase

Axxess XY exemplifies the advantages of GH10 xylanases in poultry nutrition. Its ability to efficiently act on both soluble and insoluble arabinoxylans makes it a versatile feed enzyme. The enzyme’s high thermostability ensures efficient enzyme activity in the gut and subsequent optimum nutrient utilization under challenging processing conditions, promoting gut health and maximizing performance.

Key Benefits of Axxess XY

1. Enhanced Nutrient Utilization:

   By unlocking nutrients trapped in NSPs, Axxess XY promotes better feed conversion ratios (FCRs).

2. Improved Gut Health:

   Reducing the digest’s viscosity reduces gut health challenges and predisposition to gut infections. Further, the short-chain oligosaccharides released by Axxess XY support beneficial gut microbiota, improving digestive health.

3. Economic Efficiency:

   Enabling the optimum use of high-fiber, cost-effective, locally available feed ingredients without compromising performance makes Axxess XY an asset for profitability.

In a recently conducted 42-day trial at a commercial farm, Axxess XY maintained the average body weight of broilers with a 100 kcal/kg reduction in metabolizable energy while significantly reducing feed cost/kg body weight. The diets were based on corn, DDGS, and soybean meal.

Figures 4 and 5: Body weight and cost of feed in broilers fed a diet reduced by 100 kcal/kg in metabolizable energy compared to a standard diet without Axxess XY

Conclusion

Xylanase exemplifies how feed enzymes can transcend their traditional role in feed cost reduction to support enhanced gut health. Xylanase supports reduced antimicrobial use in poultry production by improving nutrient utilization, reducing digesta viscosity, and fostering healthy microbiota. Its integration into comprehensive gut health management strategies offers a sustainable pathway to combat AMR and ensure the long-term viability of poultry farming. By targeting NSPs, these enzymes enhance nutrient digestibility, reduce feed costs, and support sustainable production practices.

GH10 xylanases, particularly Axxess XY, stand out for their superior substrate specificity, catalytic efficiency, and thermostability. By incorporating Axxess XY into feed formulations, poultry producers can unlock the full nutritional potential of feed ingredients, ensuring optimal performance and profitability. As the poultry industry continues to evolve, adopting advanced enzyme technologies like Axxess XY represents a strategic step toward sustainable and efficient animal nutrition.

References:

Baker, J.T.; Duarte, M.E.; Holanda, D.M.; Kim, S.W. Friend or Foe? Impacts of Dietary Xylans, Xylooligosaccharides, and Xylanases on Intestinal Health and Growth Performance of Monogastric Animals. Animals 2021, 11, 609.

Choct, M., and G. Annison. “Anti‐nutritive Effect of Wheat Pentosans in Broiler Chickens: Roles of Viscosity and Gut Microflora.” British Poultry Science 33, no. 4 (September 1992): 821–34. https://doi.org/10.1080/00071669208417524.

Liu D, Guo S, Guo Y. Xylanase supplementation to a wheat-based diet alleviated the intestinal mucosal barrier impairment of broiler chickens challenged by Clostridium perfringens. Avian Pathol. 2012;41(3):291-8.

Matthiesen, Connie F., Dan Pettersson, Adam Smith, Ninfa R. Pedersen, and Adam. C. Storm. “Exogenous Xylanase Improves Broiler Production Efficiency by Increasing Proximal Small Intestine Digestion of Crude Protein and Starch in Wheat-Based Diets of Various Viscosities.” Animal Feed Science and Technology 272 (February 2021): 114739. https://doi.org/10.1016/j.anifeedsci.2020.114739.

Teirlynck, E.; Haesebrouck, F.; Pasmans, F.; Dewulf, J.; Ducatelle, R.; van Immerseel, F. The cereal type in feed influences Salmonella enteritidis colonization in broilers. Poult. Sci. 2009, 88, 2108–2112.

Conference Report

Heat stress poses a significant challenge to pig production, particularly in Asia, due to the region’s warm and humid climate. In the following, Dr. Merideth Parke, Global Application Manager Swine at EW Nutrition, discusses effective management strategies to mitigate the adverse effects of heat stress on pig health and productivity.

Understanding Heat Stress

Pigs are particularly vulnerable to heat stress due to their limited ability to dissipate heat. “This is because they lack functional sweat glands, have relatively small lungs, a thick subcutaneous fat layer, and a narrow thermoneutral zone. The pigs’ thermoneutral or ‘comfort’ zone varies by age and weight. For instance, sows require 18-22°C, grow-finish pigs less than 25°C, while newborn piglets need a much warmer 35°C,” she explained.

Furthermore, today’s lean and efficient pigs have higher metabolic demands and produce more body heat, making them more susceptible to heat stress than pigs from the 1980s.

Symptoms of heat stress include:

• Increased respiration rates (>50/minute)
• Elevated rectal temperature (>39.5 oC)
• Decreased feed intake
• Reduced growth rates
• Lower reproductive performance
• Lower reproductive performance

Pigs naturally reduce their feed intake as a response to heat stress, which is a mechanism to decrease metabolic heat production from digestion. For example, research on sows has shown that for each 10°C increase between 25-27°C at 50-60% relative humidity, they reduce their feed intake by 214 g/day.

Managing Heat Stress

Managing heat stress is complex. It requires a combination of solutions specific to each production system. Additionally, it must be considered that heat stress is not only about temperature. Its impact can be exacerbated by relative humidity, which hinders heat dissipation through evaporation. The heat index chart below demonstrates the relationship between temperature, humidity, and comfort levels for a grow-finish pig. Pigs require an environment where the heat index is within the thermoneutral zone, enabling them to shed heat and maintain efficient feed utilization and growth.

While we often initially look to nutritional interventions, such as reducing dietary crude protein levels, increasing fats, or adding feed additives such as betaine, the effectiveness of these heat mitigation strategies is limited if the pigs are not eating well. Therefore, we must first focus on environmental management to reduce external heat absorption and increase heat load shedding. Pigs with the highest metabolic demands – lactating and gestating sows and finisher pigs – are especially susceptible to heat stress and should be given priority.

Several strategies to effectively manage heat stress can be used:

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1. Misters and sprinklers

   Misters or sprinklers can help cool pigs through evaporation. However, these should be used strategically – running them for short periods followed by breaks – to maximize cooling effects without creating excessive moisture and wet conditions that could lead to other health issues, such as skin lesions or respiratory problems.

   However, water-based cooling systems can inadvertently raise the heat index in humid environments. When water is sprayed into a humid environment, it will further increase the moisture levels in the air, exacerbating the heat stress situation. If humidity is too high, alternative cooling methods, such as evaporative cooling pads or high-pressure fogging systems, may be more effective.

   Snout and flank drip systems deliver water directly onto the pig’s body, mainly targeting areas more sensitive to heat. This localized approach enables heat dissipation without excessively increasing humidity in the surrounding environment.

2. Ventilation and airflow

   Increased air movement, combined with misting or sprinkling (in low-humidity environments), can enhance the cooling effect by enhancing evaporative and convective heat loss. This combination helps reduce the temperature the pigs ‘feel’, making them more comfortable.

   Producers should assess their ventilation systems and consider modifications to improve air circulation. This can be achieved by installing additional fans. However, the fans must be maintained – clean fan blades and louvers can increase efficiency by 30%. Furthermore, it must be evaluated if there are dead spots and drafts at the pig level, not along the walkways.

   Using suspended ceilings can effectively reduce the airspace that needs cooling and can lead to lower energy costs for cooling systems.

3. Housing and surroundings

   Adding insulation to roofs and walls can help reduce heat transfer inside the pig housing. Applying reflective coatings (such as white paint) to rooves and walls can help deflect solar radiation, reducing heat accumulation inside the shed by several degrees.

   Dense vegetation surrounding a piggery can provide shade and reduce reflective heat. However, it can also obstruct airflow and trap moisture, increasing local humidity and exacerbating the pigs’ heat index and heat stress.

4. Drinking water

   Providing fresh, chilled drinking water (10°C) is a highly effective method for mitigating heat stress in pigs and increasing feed intake to improve overall performance. Insulating header tanks and water pipes can help to maintain cool temperatures.

   Regular checks on water supply systems are essential to ensure they function correctly and provide adequate flow rates to the end of the line. For example, lactating sows need a flow rate of 4 L/minute.

5. Stocking density and body condition

   Higher stocking densities can exacerbate heat stress in pigs. Increased animal density leads to higher ambient temperatures due to the combined metabolic heat produced by the animals and reduced airflow at the pig level. Lower stocking densities can allow pigs to manage their body temperature better.

   Pigs with higher body condition scores (more body fat) may be more susceptible to heat stress. Excess fat can hinder effective heat dissipation, making it more difficult for these pigs to regulate their body temperature during hot weather.

6. Monitoring and evaluation

   Continuous monitoring of temperature, humidity levels, and airflow is vital to adjust cooling strategies as necessary. A common mistake when monitoring the pigs’ thermal environment is placing sensors in walkways at head height for workers because they are easier to read than at pig level in the pens. Sensors should be positioned in several locations throughout the shed. Regardless of sensor readings, stockpersons need to observe behavioral changes that provide immediate insights into the welfare and comfort of pigs during high-temperature periods.

7. Husbandry

   Pigs must be regularly observed for signs of heat stress, such as rapid breathing, reduced activity and feeding, lateral recumbency, and changes in vocalization. Aggressive behaviors may increase among pigs during heat stress as they compete for cooler spaces and water. Early detection of behavioral changes allows for timely interventions.

   “Schedule feeding during cooler parts of the day, such as early mornings or late evenings. This practice helps minimize additional heat production from digestion during peak temperatures”, according to Dr. Parke.

   “When moving pigs, especially pregnant sows, to the farrowing room, do so during the coolest times of the day and allow them to walk at their own pace.”

Conclusion

In conclusion, in the first run, each aspect of a production system must be critically evaluated, and existing housing or husbandry procedures must be modified to reduce the severity of the adverse effects of high temperatures on pig health and performance.

EW Nutrition’s Swine Academies took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Merideth Parke, Global Application Manager, Swine, was one of the highly experienced speakers of EW Nutrition. She is a veterinarian who strongly focuses on swine health and preventive medicine.

Conference Report

Essential oils, secondary plant compounds, phytogenics – all these expressions can be found in the context of animal feed. In the following, Dr. Sabiha Kadari, Regional Technical Director Southeast Asia/Pacific at EW Nutrition, will show the difference between essential oils and phytomolecules and the science behind phytogenics.

Essential oils and phytomolecules– not the same

Let us first show what are essential oils using the example of oregano oil. Essential oils are extracted from plants and unpurified mixes of different phytomolecules. The raw oregano oil extract contains carvacrol, thymol, P-cymene, and several other phytomolecules. The concentration and composition of these phytomolecules can vary significantly, depending on factors such as geographical origin, seasonal variations, plant part, plant growth stage and harvest time, extraction methods, and post-harvest processing. As a result, there can be significant batch-to-batch variations, resulting in differences in animal performance. Furthermore, there is the potential for the presence of undesirable contaminants.

In contrast, phytomolecules are the active ingredients in essential oils or other plant materials. They are clearly defined as one active compound (IUPAC name/CAS number) by their unique chemical structures, such as carvacrol. By focusing on specific active compounds, standardized products don’t have batch-to-batch variation, enhancing consistent animal performance.

Stringent screening processes

To yield the best phytogenic formulations for animal production, a rigorous screening process is required:

The initial screening process consists of ensuring the bioactives are generally recognized as safe (GRAS) by the US Department of Agriculture and approved by the European Food Safety Authority (EFSA). This step is crucial to ensure that any compounds used in formulations do not pose health risks to animals or humans.

In addition to being selected for their chemical-physical properties, which play a significant role in determining how well the phytogenics will perform in various applications, and a thorough cost-benefit analysis, the phytogenics are mapped for their following biological activities.

Antioxidant

Phytomolecules exert their antioxidant effects through various mechanisms, including scavenging free radicals. The ORAC (Oxygen Radical Absorbance Capacity) test is widely regarded as a gold standard for measuring the antioxidant potential of phytomolecules. It quantitatively assesses the ability of compounds to scavenge free radicals, providing a reliable comparison against a known standard, specifically Trolox, a vitamin E analog. Trolox has well-documented antioxidant properties, making it a reliable benchmark for evaluating the effectiveness of other antioxidants.

Antimicrobial

Incorporating a comprehensive approach to testing the antibacterial properties of phytogenics is essential for developing effective feed additives. The antibacterial properties should not only be tested against harmful enteropathogenic bacteria, such as Clostridium perfringens, E. coli, and Salmonella. It should also be evaluated if beneficial species such as Lactobacilli, the proliferation of which is wanted, are preserved.

By evaluating both pathogenic and beneficial bacteria, researchers can ensure that phytogenic formulations support optimal gut health and reduce the reliance on antibiotics.

Anti-inflammatory

Anti-inflammatory properties also help to modulate the gut-associated immune system and mitigate excessive immune response so that animals can allocate more energy towards growth and production. This shift is vital for optimizing feed conversion ratios and overall performance.

Dr. Kadari noted that “EW Nutrition uses nuclear factor kappa beta (NFkß), which regulates the expression of various pro-inflammatory cytokines, and interleukin 6 (pro-inflammatory) and 10 (anti-inflammatory) cytokines as biomarkers, for measuring anti-inflammatory activity. A reduction in NFkß and the ratio of IL-6/ IL-10 indicates a decrease in inflammatory response.”

Anti-conjugation

Conjugation is a common mechanism of horizontal gene transfer that is instrumental in spreading antibiotic resistance between bacteria. “Most resistance genes are found on mobile genetic elements named plasmids and primarily spread by conjugation,” explained Dr. Kadari.

Cell stress of bacteria modulates the conjugation frequency. Among these stressors are antimicrobial phytogenics. The goal is to keep the conjugation frequency below the one that could occur under unchallenged conditions.

Figure 1: High throughput screening allows EW Nutrition researchers to quickly conduct millions of chemical, genetic, or pharmacological tests

Delivery mechanism

Lastly, to optimize the benefit of the selected phytogenics and deliver consistent results, the substances must be protected by, e.g., encapsulation to ensure homogenous distribution in feed and thermostability in pelleted feed. A special delivery system provides for the targeted release of the active ingredients within the organism, specifically ensuring that these compounds are effectively utilized within the body rather than eliminated through the feces. This is crucial for optimizing their benefits in animal production.

Phytomolecules are an essential support in antibiotic reduction

“Phytogenics are increasingly recognized as effective alternatives in antimicrobial reduction programs. The combination of stringent screening processes alongside rigorous in vitro and in vivo testing is essential for ensuring that phytogenics deliver optimal and consistent performance in animal production,” noted Dr. Kadari.

EW Nutrition’s Swine Academies took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Sabiha Kadari, Regional Technical Director at EW Nutrition SEAP, was one of the highly experienced speakers of EW Nutrition. With expertise in feed cost optimization, feed additive management, audits, and lab support, she provides customized technical solutions and troubleshooting challenges for customers.

Conference Report

Achieving high performance and superior meat quality with preferably low investment – and here, we speak about feed costs, which account for up to 70% of the total costs – is a considerable challenge for pig producers. The following will focus on the effects of genetic enhancements and nutrient quality on overall pig performance.

Effect of body weight and gender on protein deposition

Based on Schothorst Feed Research recommendations for tailoring nutritional strategies to enhance feed efficiency and overall productivity, the following facts must be considered:

• Castrates, boars, and gilts have significantly different nutritional requirements due to variations in growth rates, body composition, and hormonal influences. For instance, testosterone significantly impacts muscle development and protein metabolism, increasing muscle mass in males. In contrast, ovarian hormones may inhibit muscle protein synthesis in females, contributing to differences in overall protein deposition. Boars, therefore, require higher protein levels to support muscle growth. Castrates typically have a higher FCR compared to gilts and boars due to higher feed intake. Split-sex feeding allows for diet adjustments to optimize growth rates and reduce feed costs per kilogram gained.
• Different body weight ranges: because puberty is delayed in modern genetics, we can produce heavier pigs without compromising carcass quality. Given that a finisher pig with 80-120 kg bodyweight consumes about half of the total feed of that pig, Dr. Fledderus concluded that extra profit could be realized with an extra feed phase diet for heavy pigs. Implementing multiple finisher diets can help reduce feed costs by allowing for lower nutrient concentrations, such as reducing the net energy and standardized ileal digestible lysine in later phases, without compromising performance.

Decision-making according to feedstuff prices

Least cost formulation is commonly used by nutritionists to formulate feeds for the lowest costs possible while meeting all nutrient requirements and feedstuff restrictions at the actual market prices of feedstuffs. However, diet optimization is more complex. The real question is, “How do you formulate diets for the lowest cost per kilogram of body weight gain?” You must always consider your specific situation, as economic results vary greatly and depend mainly on the prices of pork and feed and pig growth performance (e.g., feed efficiency, slaughter weight, and lean percentage).

How can you optimize your feeding strategy? Reducing net energy (NE) value will result in more fiber entering the diet. This makes sense if fiber by-products are cheaper than cereals. In contrast, an increase in the NE value will increase the inclusion of high-quality proteins and synthetic amino acids. It will use more energy from fat and less from carbohydrates.

The effects of diet composition on meat quality and fat composition also need to be considered.

How can nutrition improve meat quality?

Nutritional strategies not only improve the sensory attributes of pork but also enhance its shelf life, ultimately leading to higher consumer satisfaction and better marketability. Some of the factors Dr Fledderus considered included:

Improving fat quality

The source of dietary fat significantly impacts the quality of pork fat. Saturated fats tend to produce firmer fat, while unsaturated fats can lead to softer, less stable fat deposits. Diets high in unsaturated fats are more prone to lipid oxidation, negatively affecting shelf life and overall meat quality. The deposition of polyunsaturated fatty acids is only from dietary fat. Saturated fats in pork, partly originates from dietary fat and are also synthesized de novo. So, the amount of polyunsaturated fatty acids in pork depends on the content and composition of dietary fat, which can negatively affect the shelf life and perception of pork meat.

The iodine value (IV) is a measure of the degree of unsaturation in fats. A higher IV indicates a higher proportion of unsaturated fatty acids, leading to softer fat. Pork fat with an IV lower than 70 is considered high quality, as it tends to be firmer and more desirable for processing.

As per the American Oil Chemists Society, IV is calculated as:

IV = [C16:1] × 0.95 + [C18:1] × 0.86 + [C18:2] × 1.732 + [C18:3] × 2.616 + [C20:1] × 0.785 + [C22:1] × 0.723

(brackets indicate concentration (%) of C16:1 palmitoleic acid, C18:1 oleic acid, C18:2 linoleic acid, C18:3-linoleic acid, C20:1 eicosenoic acid, C22:1 erucic acid per crude fat)

Implications

Dr. Fledderus concluded that the pigs’ nutritional requirements are dynamic and influenced by factors such as required meat and fat quality, heat stress, slaughter weight, and genetic developments. Tailoring diets based on gender and body weight is crucial for optimizing protein deposition. Accurate information is essential to formulate diets that achieve optimum economic results, not just the least cost.

Continuous monitoring of feedstuff prices and nutritional content allows for timely adjustments in diet formulations, ensuring that producers capitalize on cost-effective ingredients while maintaining nutritional quality.

EW Nutrition’s Swine Academy took place in Ho Chi Minh City and Bangkok in October 2024. Dr. Jan Fledderus, Product Manager and Consultant at the S&C team at Schothorst Feed Research, with a strong focus on continuously improving the price/quality ratio of the diets for a competitive pig sector and one of the founders of the Advanced Feed Package, was a reputable guest speaker in these events.